Functional characterization of transport proteins using conventional electrophysiology can be challenging, especially for low turnover transporters or transporters from bacteria and intracellular compartments. Solid-supported membrane (SSM)-based electrophysiology is a sensitive and cell-free assay technique for ...
[16]Antje P ,Thomas L ,Felix B .Correction: Solid-Supported Membrane (SSM)-Based Electrophysiology Assays Using Surface Electrogenic Event Reader Technology (SURFEsup2/sup R) in Early Drug Discovery.[J].Current protocols,2023,3(4):e755-e755. [17]Antje P ,Thomas L ,Felix B .Solid-Suppo...
Current Protocols published by Wiley Periodicals LLC.PROTOCOL GROUP 1: Sample preparation for SSM-based electrophysiology assaysSupport Protocol 1: Production of cell batchesSupport Protocol 2: Simple isolation of cell membranesAlternate Protocol 1: Isolation of cell membranes with sucrose gradient pre-...
This chapter focuses on how the SSM-based electrophysiology has contributed to unraveling the transport mechanism of two prominent members of the P-type ATPase family, that is, the sarcoplasmic reticulum Ca-ATPase and the human Cu-ATPase (ATP7A and ATP7B)....
Andre Bazzone aAlexander Krner b cMelanie Meincke aManan Bhatt aSrujan Dondapati bMaria Barthmes aStefan Kubick b d eNiels Fertig a
SSM-based electrophysiology of F267C NhaA.Dudu AlkobyAbraham RimonMaral BurdakMiyer PatinoRuizOctavian CălinescuKlaus FendlerEtana Padan
Solid supported membrane (SSM)-based electrophysiology is useful for functional characterisation of ligand-gated ion channels like nAChRs, as charge translocations via capacitive coupling of the supporting membrane can be measured. By varying the agonist (carbamoylcholine) concentration, different functional...