使用Long ssDNA Preparation Kit (LsODN Preparation Kit)制备的1,500 base和3,000 base的长链ssDNA。 将目的dsDNA片段(1,500 bp或者3,000 bp)在pLSODN -1或pLSODN-3的MCS(Nt.BspQI位点与Nb.BsrDI位点之间)克隆后,使用切口酶(Nt.BspQI和Nb.BsrDI)对其处理,在目的片段的两端出现切口。变性后,样品经琼脂糖凝...
16.6 Species Other PMID 39179589 Primase promotes the competition between transcription and replication on the same template strand resulting in DNA damage - PMC Journal Nature communications Published 2024 IF 16.6 Species Arabidopsis thaliana PMID
Long ssDNA Preparation Kit for 10kb 产品编号产品名称产品规格产品等级产品价格
试剂盒采用双抗体一步夹心法酶联免疫吸附试验(ELISA)。往预先包被抗体的包被微孔中,依次加入标本、标准品、HRP标记的检测抗体,经过温育并彻底洗涤。用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中呈正相关。用酶标仪在450nm 波长下测定吸光度(OD 值),计算...
(Tanon 5200, Shanghai, China). Besides, the ODN pool bound to the beads was digested using proteinase K (Servicebio, Wuhan, China) at 37 °C for 3 h and then extracted with phenol-chloroform for further ssDNA library preparation. The protein of interest was eluted in 40 μl 2 ...
NGS Library Preparation Kit NGS library preparation enable efficient conversion of DNA/RNA samples into indexed libraries for subsequent target enrichment process or direct sequencing. Hybridization Capture Products Efficiently capture and enrich target DNA sequences for precise analysis. Amplicon ...
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Strand directionality is retained by denaturing the DNA before library preparation and ligating the first adaptor directly at the 3′ of ssDNA, using Adaptase. This methodology has been applied successfully to discriminate between leading or lagging strands in DNA replication (Yu et al., 2018) and...
Preparation of RAD52 The sequence encoding human RAD52 was ordered from GenScript as codon-optimized for E. coli, and was cloned into pMALT-P (a kind gift of the Kowalczykowski laboratory, UC Davis) using BamHI and PstI restriction sites. Additionally, a His-tag was inserted before the ...
Within the first round of Cell-SELEX, the library was prepared by the addition of 100 μL of 100 μM (10 nmol) DNA library to 270 μL of aptamer binding buffer. The pool was then heated to 95 °C for 5 min and snap cooled on ice until ready to use. For the preparation of RBCs...