Long ssDNA Preparation Kit (LsODN Preparation Kit),通过简单的操作,便可获得含有目的序列的高纯度长链ssDNA。 ◆特点 ● 不含有变异或末端缺失,可制备1,500 base或者最长到3,000 base的长链ssDNA。 ●全程操作与普通的dsDNA片段制备法相同,无需准备特殊的机器以及试剂。 ●通过附加的Denaturing Gel-loading Buffer...
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experiments involving CRISPR/Cas9 technology or other genome editing tools. This kit provides a simple and fast method for converting a dsDNA PCR product of any sequence into ssDNA via selective digestion of either the sense or the antisense strand. Each kit allows for preparation of 50 ssDNA ...
The kit is intended for quantification of single-stranded DNA (ssDNA) with fluorometer. With this kit you can measure concentration of either oligonucleotide or long ssDNA. The kit includes concentrated QuDye ssDNA Reagent, dilution buffer, and ssDNA quantitative standards (0 and 20 ng/uL). For ...
Genomic DNA preparation and sequencing: Genomic DNA (gDNA) was isolated using the Blood & Cell Culture DNA Mini (< 5e6 cells) Kits (Qiagen, cat. no. 13323) as per the manufacturer’s instructions. The gDNA concentrations were quantified by Nanodrop. For PCR amplification, 1 μg of gD...
ssDNA or target ssRNA.cTarget recognition of the gRNA-bound EcAgo activates EcbAgaN. About 300 ng genomic DNA was treated with EcbAgaN. Aliquots of the reaction were also supplemented with EcAgo, gRNA and/or target ssDNA or other oligonucleotides. TD: target ssDNA, NTD: nontarget ssDNA,...
We also show that Sgs1 can unwind duplex DNA harboring a nick, in a manner dependent on a species-specific interaction with the ssDNA-binding factor replication protein A (RPA). These biochemical systems and results will be valuable for guiding future endeavors directed at delineating the ...
long ssDNA flaps behind the forks through Pif1 and fork speed. We suggest that Dna2 offsets the strand displacement activity mediated by the lagging strand polymerase and Pif1, processing long ssDNA flaps to prevent DDR activation. We propose that this Dna2 function has been hijacked by Break...
Su- pernatants were collected and pre-cleared with Dynabeads G in ChIP lysis buffer with the supplement of 100 µg BSA and 100 µg ssDNA. Then, the pre-cleared cell lysates were used for ChIP with 2 µg CTCF antibody (Millipore) for overnight incubation at 4 °C. The beads ...
(C)CCAT1-LregulatesMYCexpression at the transcriptional level. A crude preparation of nuclei was subjected to nuclear run-on assay under the indicated conditions in HT29 cells. Nascent transcription ofMYCdetected from scramble ASO-treated nuclei was defined as one.(D)Overexpression ofCCAT1-L in ...