. This point mutation was validated to interrupt tri-snRNP assembly in human HCC cells. RNA immunoprecipitation (RIP) results showed that the decreased U4 and U6 snRNAs were co-purified with the C139A mutant, which indicated specific defects in tri-snRNP biogenesis (Figs.3B,S5–7B). Unlike wi...
We made a kinase dead DRAK2 (DRAK2-S12A) by mutation of Ser12 to alanine, an autophosphorylation and key site for kinase activity. Through a new set of experiments, we found that DRAK2-S12A also binds with SRSF6, suppresses SRSF6 phosphorylation, and exacerbates the PAOA-induced lipid ...