Phenomenon explanation:The possible reasons that the actual band size differs from the predicted are as follows:1.Splice variants: Alternative splicing may create different sized proteins from the same gene.2. Relative charge: The composition of amino acids may affects the charge of the protein.3....
1.Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: ...
A number of duplications of the X-linked SRPK3 gene were identified (Figure 6B), which is likely explained by frequent X-chromosome duplications in developmental disorders. Interestingly, several point mutations within the SRPK3 kinase domain (Figure 6B, bottom) have been reported in X-linked ...