1、比较miRNA及其靶基因的表达模式,联合sRNA-seq和RNA-Seq数据进行分析。正如预期的那样,大多数靶基因的表达趋势与相应的miRNA相反。 2、dcl1-9突变体中,DEGs数量大幅降低,突出dcl1-9对抗TCV反应起负作用。此外,PPR基因可能在植物抗病毒防御中发挥重要作用。 参考文献: Analyses of ...
We show that 5XP-seq not only enriches phosphorylated miRNA and piRNA but successfully discriminates these sRNA from all other sRNA species. We further demonstrate the importance of this strategy by successful inter-species validation of sRNAs that would have otherwise failed, including human to ...
reads长度是150bp，是用磁珠把所有的有polyA的mRNA都调出来，一个miRNA测序，是切胶回收22-50bp的sRNA...
Integrated RNA-seq and sRNA-seq analysis identifies novel nitrate-responsive genes in Arabidopsis thaliana roots 来自 EBSCO 喜欢 0 阅读量: 210 作者:EA Vidal,TC Moyano,G Krouk,MS Katari,M Tanurdzic,W Mccombie,GM Coruzzi,RA Gutiérrez
The ncPRO-seq pipeline supports input read sequences in fastq, fasta and color space format, as well as alignment results in BAM format, meaning that sRNA raw data from the three current major platforms (Roche-454, Illumina-Solexa and Life technologies-SOLiD) can be analyzed with this ...
viral defense in TCV-infected Arabidopsis thaliana Chao Wu1, Xinyue Li2, Song Guo3 & Sek-Man Wong1,3,4,5 In order to identify specific plant anti-viral genes related to the miRNA regulatory pathway, RNA-Seq and sRNA-Seq were performed using Arabidopsis WT and dcl1-9 mutant ...
Subramanian D, Bhasuran B, Natarajan J. 7 October 2018. Genomic analysis of RNA-Seq and sRNA-Seq data identifies potential regulatory sRNAs and their functional roles in Staphylococcus aureus. Genomics https://doi.org/10.1016/j.ygeno.2018.09.016....
利用PBOX-sRNA-seq,研究人员可以从严重降解的RNA样本(RIN=4.15)以及AGO-RIP样品中高效克隆出全长的miRNA和siRNA。对拟南芥hen1-2弱突变体进行分析发现,HEN1的部分缺失更容易引起miRNA甲基化水平的下降。与预期一致,大量截短和加尾的miRNA(被认为是非甲基化的miRNA)在PBOX-sRNA-seq中不能被克隆。然而,相较于长...
Investigation of genes associated with petal variations between diploid and autotetraploid in Chinese cabbage (Brassica rapa L. ssp. pekinensis) by RNA-seq and sRNA-seq 来自 Semantic Scholar 喜欢 0 阅读量: 169 作者:F Shi,Y Wang,S Huang,S Dong,H Feng ...
测序方案:对所取样本进行mRNA-seq,sRNA-seq及降解组。并通过RT-qPCR和Northernblot进行验证。 结果 1 营养组织和生殖组织对热胁迫的不同的转录反应 对常温下生长的玉米自交系B73在V3期和R1期分别进行了短(2h)或长(48h)的热胁迫(38°C)处理。热胁迫后,V3期玉米新生叶片比对照叶片卷曲,叶片中叶绿素含量下降。在...