MaxQuant default settings were used except for variable modifications for N-terminal protein acetylation, methionine oxidation, and lysine acetylation, and a static modification for carbamidomethyl cysteine. Peptide and protein identifications were performed against Uniprot human protein database (version ...
The expression levels of SARS-CoV-2 N protein, SREBP cleavage-activating protein (SCAP), mature N-terminal SREBP2, NLRP3, procaspase-1, cleaved caspase-1, IL-1尾 and IL-18 were examined by western blotting. The expression levels of SREBP2, HMG-CoA reductase, HMGCS1, l...
To further identify Srebp2-bound genes whose transcription is specifically regulated by Qki-5, we performed cellular pathway enrichment analysis of the overlapping promoter-bound peaks (n= 9,032) in Qki-5, Srebp2, and Pol II ChIP-seq data in NLPCs (Fig.5a). We found that cholesterol biosy...
NFATc1 is considered the terminal switch that regulates osteoclast differentiation by promoting the expression of numerous osteoclast-specific genes involved in osteoclast fusion such as dendritic cell-specific transmembrane protein (DC-STAMP), V-ATPase V0 domain subunit d2 (ATP6V0d2), and bone ...
JNK C-Jun N-terminal kinase LDL Low-density lipoprotein MMP Mitochondrial membrane potential mTOR Mammalian target of rapamycin MTT Methylthiazolyldiphenyl-tetrazolium bromide NAFLD Non-alcoholic fatty liver disease ORO Oil red O PA Palmitic acid PKR Protein kinase R ROS Reactive oxygen species RT Room...
we report the first observation of SREBP-2 C-terminal fragment in COVID-19 patients'blood and propose SREBP-2 C-terminal fragment as an indicator for determining severity.We confirmed that SREBP-2-induced cholesterol biosynthesis was suppressed by Sestrin-1 and PCSK9 expression,while the SREBP-2...
Notably, we demonstrate that the C-terminal domain (CTD) of SREBP2, rather than its N-terminal domain (NTD), mediates this inhibition by interacting with HBx and promoting its extracellular secretion, thus reducing nuclear HBx accumulation. These findings reveal a novel regulatory p...
c-Jun N-terminal kinase 2cell death-inducing DNA fragmentation factor-α-like effectormicroarraysterol regulatory element-binding protein-1cde novo lipogenesissmall interfering RNAInsulin plays important roles in apoptosis and lipid droplet (LD) formation, and it is one of the determinants involved in...
It was found that c-Jun N-terminal kinases (JNK)/mammalian target of rapamycin (mTOR)/sterol regulatory element-binding protein 1c (SREBP-1c) signaling participate in the regulation of de-novo lipogenesis. In conclusion, lipid deposition in abalone was affected by dietary lipid levels through ...
Once nuclear, the N-terminal portion of the protein comprising the DNA binding and transactivation domains is subject to further regula- tion, including FBWX7-dependent ubiquitination [21, 22]. Interest- ingly, binding of FBWX7 to SREBP depends on phosphorylation of the conserved CDK2-...