Nanjing, China) and amplified through qPCR using qPCR Master Mix (Vazyme, Nanjing, China). The primers used were: SREBP1 forward: 5′-CGC TCC TCC ATC AAT GAC A-3′, reverse:
Primer direction (F = forward, R = reverse) and hybridization position on the sequence. 2 Efficiency of amplification as calculated by LinRegPCR (www.linregpcr.nl). 3 Cycle quantitation as calculated by LinRegPCR for the pAd-GFP group only, where Ad-GFP = recombinant adenovirus of green fluo...
Allele-specific primers and probes that hybridize to these regions, and/or which comprise at least one polymorphic site are also provided. The polynucleotides, primers, and probes of the present invention are useful in phenotype correlations, paternity testing, medicine, and genetic analysis. Also...
RNA extraction and RT-qPCR were performed as described previously24. Primers used were: FASN: Fw 5′-TCCGAGATTCCATCCTACGC-3′, Rv 5′-GCAGCTGTGACACCTTCAGG-3′, ACLY: Fw 5′-TGTAACAGAGCCAGGAACCC-3′, Rv 5′-CTGTACCCCAGTGGCTGTTT-3′, ACACA: Fw 5′-TGAACTTCACACAGGTAGTCTGCC-3′...
Quantitative real-time PCR (TaqMan®) was performed using 25 ng of reverse-transcribed total RNA with 300 nmol/l sense and antisense primers, 100 nmol/l probe (Table 1), and 12.5 μl of Master Mix (Qiagen, Crawley, UK) in a total volume of 25 μl in an ABI PRISM 7700 sequence ...
表 1 引物序列 Table 1 The sequences of primer 引物 序列(5′-3′) Primers Sequences VF CTCGAGTCTAGAGGGCCCGT 产物长度 Size 5.4 kb VR GATGAGTTTTTGTTCCATGGTGGCAAGCTTA AGTTTAAACGCTAGCCA 1F CCATGGAACAAAAACTCATCTCAGAAGAGG 1170 bp ATCTGGACGAGCCACCCTTCAAC 1R TGAGCTTCTGGTTGCTGTGCT 2F AGCACA...
The nucleotide sequences of primers are summarized in Supplementary Table 1. Plasmids, small interfering RNAs, and transfection Flag-tagged rat SREBP1c encoding the amino-terminal fragment of the wild-type SREBP1c protein with maximal activity (1–403 amino acids) was generated82,83. In ...
The primer sequences included the following: GK5 sense 5’-TGAGGGACACAAGCCACAAT-3′, GK5 anti-sense 5’-GGAAGCAGCACTCTCCAAAC-3′. SCD1 sense 5’-GTACCGCTGGCACATCAACTT-3′, SCD1 anti-sense 5’-TTGGAGACTTTCTTCCGGTCAT-3′. β-actin sense 5’-CTGGCACCCAGCACAATG-3′, β-actin ...
China) were used to extract RNA following the manufacturer’s protocol. 1 ng RNA was used for reverse reaction and then used for qPCR reaction with 2 × Universal SYBR Green Fast qPCR Mix (Cat: RK21203, Abclonal, China). ACTB served as an internal control. The primer sequences [25...
SERBP1 Primer:A:5'CGTTCGCCATAACCAAG TAGAG 3',B:5'GGCGATGCTGTACACTGTTGA 3',产物长度:126 bp;ACCαPrimer:A:5'AGCGCTA CCGTTCCTCTATCAA 3',B:5'GCTGTAAGAAGCGG ATGTAGTCG 3',产物长度:118 bp;SCD1 Primer:A:5'CATCGCCAACACCATGGCATT3',B:5'TCTGGAA CATCATCACCAGCTTCTC3',产物长度:186 ...