species reactivity rat, human 浓度 ~1 mg/mL technique(s) ELISA: 1:20000immunohistochemistry: 1:50-1:100western blot: 1:500-1:1000 NCBI登记号 P36956 UniProt登记号 P36956 运输 wet ice 储存温度 −20℃ Gene Information human ... SREBF1(6720) ...
来自 NCBI 喜欢 0 阅读量: 8 摘要: Sterol responsive element-binding protein 1c (SREBP1c) is a key transcription factor for de novo lipogenesis. Although SREBP1c is expressed in pancreatic islets, its physiological roles in pancreatic β cells are largely unknown. In this study, we demonstrate...
1 材料与方法 1.2 方法 1.2.1 奶牛 SREBP1 基因的克隆 采用 TRIZOL 提取 乳腺组织的 RNA,反转录成 cDNA,根据 NCBI 公布 的奶牛 SREBP1 基因序列(NM_001113302.1),设计 引物时在 SREBP1 序列 ATG 后加入 c-myc 序列 ( GAACAAAAACTCATCTCAGAAGAGGATCTG ) 作为标签蛋白 , 采用分段克隆 PCR 方法对奶牛 ...
SREBP一1 cDNA 序列 。利用 NCBI 的 ORF Finder( Open Reading Frame Finde) 程 序预 测其 开放 阅读 框 (ORF) , 并 翻译 其 氨基 酸序 列 。利用 Protein—BLAST 同 源搜索 其 保守 结构域 ,确 定其 可能 的功 能。 1.2 蛋 白质 结构 预测 与分 析 ...
spectrophotometrically using a Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Samples were analyzed on an HiSeq2000 (Illumina). The raw sequencing data are publically available and can be accessed athttps://www.ncbi.nlm.nih.gov/sra/...
chemical, and phylogenetic functional information. (http://en.wikipedia.org/wiki/KEGG). We used it to compare genomic profiles and look for significantly differentially expressed genes. The raw RNA-seq data was uploaded to NCBI SRA database (SRA accession number: SRR13399489)....
12a). NCBI GEO dataset from HCC patients (GSE54238) indicated that ZDHHC23 expression was negatively correlated with PHF2 expression but positively correlated with the expression of SREBF1, its target genes, cell cycle-related genes, and HCC marker genes (Fig. 8a and Supplementary Fig. 12b)....
(underlined) to facilitate the ligation of cDNA product into pcDNA3.1(+) vector (Invitrogen, USA). The mouse Insig-1 primers used in this study were tested using Blast software (http://blast.ncbi.nlm.nih.gov/Blast.cgi), and did not recognize the rat Insig-1 mRNA sequence. The sequence...
引物序列检索自 NCBI⁃Nucle⁃ otide 基因库,采用 Primer5 0 软件设计,由北京赛百 胜基因有限公司合成. SOCS⁃3 mRNA 引物:5′⁃CAG CTCCAAGAGCGAGTACCAG⁃3′ 5′⁃CATGTAGTGGT⁃ GCAC CAACTTGA ⁃3′,94℃ 预变性 5 min,94℃ 变性 45 s, 59℃ 退火 45 s,72℃ 延伸 1 min,循环...
We show the average ratio of one representative spot for each regulated gene, with standard error calculated from three hybridizations and with the annotation provided by the microarray facility (Hver1.2.1_NCBI35). The data files were submitted to the databases ArrayExpress (accession number E-...