共同作者Magnus-Carsten Huppertz和Jonas Wilhelm以及同事们开发了Split-HaloTag——在特定细胞活动和荧光底物的存在下会被标记的蛋白质。荧光底物和瞬时细胞活动的结合，比如蛋白质间的相互作用或细胞内Ca2+水平的增加，可用于有理设计记录器，以捕捉稍后分析的瞬时事件。荧光底物的加入和洗脱确定了记录时段。这些步骤之前...
所有这些特性都在使用设计用于Ca2+依赖性蛋白标记(Caprola)的split-HaloTag记录器的实验中得到验证。Huppertz和Wilhelm使用Caprola记录了斑马鱼幼鱼和成年蝇的神经活动。他们还基于它们的Ca2+水平对各种细胞群体进行了排序和分析转录组。鉴于Ca2+信号的广泛使用和HaloTag标记在许多模型系统中的成功应用,Caprola有望在生物...
Split-HaloTag imaging assayAn ever-increasing number of intracellular multi-protein networks have been identified in plant cells.Split-GFP-based protein–protein interaction assays combine the advantages of in vivo interaction studies in a native environment with additional visualization of protein complex...
Using this approach, we have engineered and optimized the tag-assisted split HaloTag complementation system (TA-splitHalo) and demonstrated its versatile applications in improving the efficiency of knock-in cell enrichment, detection of protein-protein interaction, and isolation of biallelic gene edited...
Using this approach, we have engineered and optimized the tag-assisted split HaloTag complementation system (TA-splitHalo) and demonstrated its versatile applications in improving the efficiency of knock-in cell enrichment, detection of protein-protein interaction, and isolation of biallelic gene edited...
Therefore, we established the new Split-HaloTag imaging assay to enable sophisticated fluorescence imaging of protein-protein interactions. This is demonstrated using an well-characterised interaction as an example for protein-protein interaction at cellular structures: the molybdenum cofactor biosynthesis ...
Using this approach, we have engineered and optimized the tag-assisted split HaloTag complementation system (TA-splitHalo) and demonstrated its versatile applications in improving the efficiency of knock-in cell enrichment, detection of protein-protein interaction, and isolation of biallelic gene edited...