第二个危害,也就是异常的剪接体本身可能会产生一些具有治病能力的多肽或者蛋白,也就是脱轨本身造成的事故,比如失控的火车会冲入村庄,造成灾难。 如果我们能在转录组数据RNA-seq中,检测找出这种治病的异常剪接,那么可能会对一些疾病的治疗提供新的视野。 2、Previous Work 既然我们已经知道了异常剪接的危害,那么如何在...
Alternative splicing signatures in RNA3seq data: Percent spliced in (PSI). Curr Protoc Hum Genet. 2015; 87:11.16.1-11.16.14. [PubMed: 26439713]Schafer, S. et al. Alternative Splicing Signatures in RNA-seq Data: Percent Spliced in (PSI). Curr Protoc Hum Genet 87, 11.16.1-11.16.14 (...
另一种对理解可能由基因产生的可变剪接产物的复杂性非常有用的方法是一种称为 RNA-seq 的方法。这种方法的工作方式与您已经了解的配对末端 DNA-seq 反应非常相似 ,但在这种情况下,起始模板必须是 mRNA。 所以你从细胞的细胞质中分离出 mRNA,这样当你得到成熟的 mRNA 时,你就可以将它们复制到 cDNA 中。 然后,...
Detection of aberrant splicing events in RNA-seq data using FRASER Aberrant splicing is a major contributor to rare disease, but detection accuracy using current methods is limited. Here, the authors develop an algorithm that detects aberrant splicing and intron retention events from RNA-seq data ...
The ubiquity of RNA-seq has led to many methods that use RNA-seq data to analyze variations in RNA splicing. However, available methods are not well suited for handling heterogeneous and large datasets. Such datasets scale to thousands of samples across
RNA-seq over microarrays, used older versions of these arrays, mainly focused on the abundance of 3′ UTRs, not of entire genes. New array platforms like the Affymetrix Human Transcriptome Arrays 2.0 (HTA) use improved methods for the quantification of transcripts. As the probes in these ...
FRASER is a tool to detect aberrant splicing events in RNA-seq data. The method is described and published inNature Communicationsand available throughBioconductor. It is also part of theDetection of RNA Outlier Pipeline (DROP). DROP is described and published inNature Protocols. ...
(TWAS) to expand the collection of candidate effector genes for T2D and related traits. In this approach, which so far has not been applied to human islet RNA-seq data, genetic effects on splicing or expression are used to impute transcript variation in cases versus controls from GWAS ...
SpliceNet recovering splicing isoform-specific differential gene networks from RNA-Seq data of normal and diseased samples 热度: nrc.2016.51-RNA splicing factors as oncoproteins and tumour suppressors 热度: 相关推荐 RNA剪接 RNASplicing Primarytranscript Mostoftheeukaryoticgenesaremosaic(嵌合体),consis...
whose transcriptome has been recently monitored by RNA-Seq in the framework of the Geuvadis Project8. The results obtained demonstrate the power of our approach. SNPs within a tested gene are about 100-fold more likely to be sQTLs for that gene that SNPs mapping to another gene consistent wit...