Splice donor and acceptor sites found in Cabb B-JI and Cabb-S strains by RT-PCR.Clément BoutonAngèle GeldreichLaëtitia RamelLyubov A. RyabovaMaria DimitrovaMario Keller
3d, the Splice acceptor/donor sites had the most ΔSplice scores close to 1, while missense and nonsense SNVs showed minimal ΔSplice scores. Interestingly, we observed a bimodal distribution of ΔSplice scores for intronic and synonymous SNVs in the pathogenic and likely pathogenic groups, with...
3d, the Splice acceptor/donor sites had the most ΔSplice scores close to 1, while missense and nonsense SNVs showed minimal ΔSplice scores. Interestingly, we observed a bimodal distribution of ΔSplice scores for intronic and synonymous SNVs in the pathogenic and likely pathogenic groups, with...
level; i.e., it attempts to recognize donor and acceptor sites in pairs, just as the spliceosome operates in the cell when it splices out an intron. note that most human genes have multiple splice isoforms, with current annotation catalogs containing 5–10 isoforms per gene. thus, an accura...
Training and testing procedure The human reference genome (GRCh38) was used to construct the dataset. We obtained the location of exons from the annotation file, took sequences centered at the right and left boundaries of exons, which correspond to donor sites and acceptor sites, as the positiv...
PMOs also have been identified to target the putative polypyrimidine tract of a cryptic splice acceptor site created by c.-32-13T>G mutation as part of the pseudoexon created in GAAintron 1, blocking the cryptic splice donor, which leads to the inclusion of exon 2. Van der Wal et...
A novel method is proposed to detect the acceptor and donor splice sites using chaos game representation and artificial neural network. In order to achieve high accuracy, inputs to the neural network, or feature vector, shall reflect the true nature of the DNA segments. Therefore it is importan...
non-coding regions (introns). Splice site is the general term for the point at the beginning (donor site) or at the end (acceptor site) of an intron. Donor and acceptor sites typically correspond to certain patterns, but the same patterns can also be found in other places in the DNA ...
These splice junctions provide experimental evidence for an executed splicing reaction using the following: (1) a paired donor and acceptor from different introns, reflecting skipping of one or more consecutive exons normally present in that transcript (Fig. 2a; exon skipping); or (2) an annotated...
The splice junction was characterized for viral mRNA from a prototype subgroup A FelV and an immunodeficiency-inducing FeLV variant. Thirty-seven of 38 partial envelope cDNAs, cloned after PCR amplification from T cells infected with either virus, utilized the same splice donor and acceptor sites ...