Liu. 1990. Myelin formation after transplantation of normal fetal spinal cord fragments or cultures into myelin-deficient rat spinal cord. J. Neurocytol. 19: 718-730.Hasegawa, M, Rosenbluth, J (1991) Transplantation of labeled fetal spinal cord fragments into juvenile myelin-deficient rat spinal ...
aLarge and dorsally located spinal cord V2a-INs (Chx10:GFP+, green) are cholinergic (ChAT+, magenta). Arrowheads indicate double-labeled neurons (Chx10:GFP+ChAT+).bA sample stack from the central canal area showing the presence of V2a-IN (Chx10:GFP+) axonal collaterals (green) close to...
After washing with PBS for 3 times, the sections were labeled with secondary antibodies, rabbit IgG-Alexa 488 (ThermoFisher Scientific), rabbit IgG-Alexa 594 (ThermoFisher Scientific), mouse IgG-Alexa 488 (ThermoFisher Scientific) and mouse IgG-Alexa 594 (ThermoFisher Scientific) for 50 min ...
(f)/+mice, in which all inhibitory neurons are genetically labeled with Tdt while all RET+ cells are labeled by a cyan fluorescent protein (CFP), and co-stained spinal cord sections with the pan-neuronal markerNeuN(Figures 1G–1I). Approximately 93.9% ± 0.7% of CFP+ cells are NeuN+,...
Fig. 1. Ependymal cells appear at mid-embryonic stage in the developing mouse spinal cord. (A) Representative coronal section images of YFP labeled ependymal cells around the central canal (dashed lines) of tamoxifen recombined FoxJ1-CreERT2::YFP. YFP expression is induced by tamoxifen at E13....
Time-dependent changes in Bolton-Hunter-labeled125I-substance P binding occurred in the dorsal horn of the spinal cord following unilateral adjuvant-induced inflammation in the hindpaw of the rat. Inflammation was characterized by measures of edema and hyperalgesia. Edema and hyperalgesia were both pr...
Our data demonstrate the presence of GFP-labeled cells in the injured spinal cord for up to 16 weeks after transplantation in the subacute SCI stage. GFP-labeled cells homed to the site of injury and were detected around white matter tracts and surviving axons. ABMC therapy in the canine ...
Purified rat Schwann cells labeled with Hoechst 33342 were transplanted into a lysophosphatidyl choline induced myelin lesion of the adult shiverer mouse spinal cord. Remyelination by grafted Schwann cells within the lesion was evidenced by codetection of Hoechst labeled Schwann cells and P0 (periphera...
Microglia proliferate extensively and accumulate at the lesion border after SCI.a–fConfocal immunofluorescence microscopy of representative spinal cord sections showing the spatio-temporal distribution of microglia (TdT, red) and astrocytes (GFAP, cyan) in an uninjuredCx3cr1creER::R26-TdTtransgenic mous...
Single-cell RNA sequencing data can unveil the molecular diversity of cell types. Cell type atlases of the mouse spinal cord have been published in recent years but have not been integrated together. Here, we generate an atlas of spinal cell types based