(b) Representative photomicrographs of cross- section of spinal cord stained with hematoxylin and eosin, Mac-1 immunohistochemisty and luxol blue stain at 28 days after injury. With increasing severity of injury, there is evidently increasing cell damage, macrophage/microglial reactivity and myelin ...
Spinal cord injury (SCI) is the result of a trauma along the spinal column that produces contusion, compression, and potential transection of the spinal cord and the interruption of afferent nerve signals from distal structures. From: Neurobiology of Disease, 2021 ...
GFP-labeled spinal cord motor neuron co-expressing NeuN.Sreenivasa R. SankavaramMikael A. SvenssonTomas OlssonLou BrundinClas B. Johansson
The spinal cord segments were cryosectioned transversely into 20 mm serial sections to examine the distribution of transplanted cells following LP. The brain was also examined in sagittal orientation and in cross-section at the level of the lateral and fourth ventricles to examine whether ...
In the present paper, a novel and simple hOC score was developed and validated to follow spinal cord cross-sectional slice quality over time in culture and after slice injury. The hOC score was also applied prior to randomly assigning slices either to SCI or control group, to support the sel...
In the spinal trigeminal nucleus caudalis, which corresponds to the dorsal horn of the cervical cord, the densest 5-HT innervation is that of lamina I (Li et al., 1997; López Costa et al., 1994), in which nociceptive primary afferents make synapses. As in the dorsal horn of spinal co...
Image Pro Plus (Media Cybernetics, Silver Spring, MD) was used to measure the tissue area of a 4.5 mm section of spinal cord with the lesion center at its midpoint. Tissue cavitation was measured in all animals to gain an estimate of the effect of the transplanted cells upon the ...
Time-dependent changes in Bolton-Hunter-labeled125I-substance P binding occurred in the dorsal horn of the spinal cord following unilateral adjuvant-induced inflammation in the hindpaw of the rat. Inflammation was characterized by measures of edema and hyperalgesia. Edema and hyperalgesia were both pr...
Crmp42/2 spinal cord exhibited 147.8% increase in immunoreactivity of 5-HT within 1–2 mm caudal to injury site when compared to controls (Fig. 6f, P , 0.05). Cross sections of spinal cord at a level 4 mm caudal to a complete transaction were also examined for 5-HT-positive profiles...
Single-cell RNA sequencing data can unveil the molecular diversity of cell types. Cell type atlases of the mouse spinal cord have been published in recent years but have not been integrated together. Here, we generate an atlas of spinal cell types based