The mixture of 69 SIRV isoform transcripts and 92 non-overlapping ERCC RNAs addresses the need for complex spike-in RNA controls that cover both, a high level of isoform complexity and a large concentration range. Together, they enable an even more comprehensive quality assessment and monitoring ...
(e.g. single cell applications). Due to their sequences being non-identical to genomic and transcriptomic database entries they can be combined with RNA from any organism (seeModular Designfor details). Since the SIRV RNAs are polyadenylated, library preparation can start from poly(A)-...
These synthetic RNAs were spiked at known molarity over a wide range into total RNA prior to construction of next-generation sequencing mRNA libraries to generate RNA-seq data. Results Leveraging a priori knowledge about replicates and molarity of each synthetic fusion transcript, we demonstrate ...
Small RNAsNormalization of high-throughput small RNA sequencing (sRNA-Seq) data is required to compare sRNA levels across different samples. Commonly used relative normalization approaches can cause erroneous conclusions due to fluctuating small RNA populations between tissues. We developed a set of sRNA...
TitleRole of d-tocotrienol and resveratrol supplementation in the regulation of micro RNAs in patients with metabolic syndrome: A randomized controlled trial CitationComplementary Therapies in Medicine2023. AuthorsSafia Fatima, Dilshad Ahmed Khan, Fozia Fatima, Muhammad Aamir, Aamir Ijaz, Ayesha Hafeez, ...
血清和血浆中主要含有小RNAs,因此无需分开纯化小RNA和大RNA组分。 使用miRNeasy Serum/Plasma Spike-In Control进行标准化 使用miRNeasy Serum/Plasma Kit纯化miRNA时,加入QIAzol Lysis Reagent后,在加入氯仿分相前,向样本中加入miRNeasy Serum/Plasma Spike-In Control。real-time RT-PCR后,合成miRNA的CT值可对样本进...
High-throughput sequencing of cDNA (RNA-seq) is a widely deployed transcriptome profiling and annotation technique, but questions about the performance of different protocols and platforms remain. We used a newly developed pool of 96 synthetic RNAs with various lengths, and GC content covering a 2...
When filtered for 41.5-fold difference, po0.01, 186 transcripts were downgulated and 76 were upregulated in the Mll1-deficient striatum, marking a 2.3-fold excess of decreased (vs increased) striatal RNAs Neuronal Mll1 regulates striatal function EY Shen et al after Mll1 ablation. This ...
the inoculum was removed, and the specimens were washed three times with PBS. Tissue cubes were maintained in DMEM/F12 medium supplemented with different concentrations of the indicated drugs for an additional 48 h. Then the tissues were harvested and the viral RNAs copies were measured by RT...
To further evaluate the in vivo antiviral role of nAbs in the rabbit sera, we injected the purified IgG from the rabbit sera intraperitoneally into hACE2 transgenic mice and Syrian golden hamsters at a dose of 5 mg IgGs/20 g body weight, and estimated the viral genomic RNAs (gRNAs)...