SIRV-Set 3 (Cat. No. 051) contains the isoform Mix E0 and the ERCC Mix 1 in equal shares. Both contribute equally to the final mass.The mixture of 69 SIRV isoform transcripts and 92 non-overlapping ERCC RNAs addresses the need for complex spike-in RNA controls that cover both, a hig...
(e.g. single cell applications). Due to their sequences being non-identical to genomic and transcriptomic database entries they can be combined with RNA from any organism (seeModular Designfor details). Since the SIRV RNAs are polyadenylated, library preparation can start from poly(A)-...
Title Role of d-tocotrienol and resveratrol supplementation in the regulation of micro RNAs in patients with metabolic syndrome: A randomized controlled trial Citation Complementary Therapies in Medicine 2023. Authors Safia Fatima, Dilshad Ahmed Khan, Fozia Fatima, Muhammad Aamir, Aamir Ijaz, Ayesha Haf...
High-throughput RNA sequencing enables the quantification of transcript abundance and the identification of novel transcripts in biological samples. These include circular RNAs (circRNAs), a family of alternatively spliced RNA molecules that form a continuous loop. However, quantification and comparison of...
Novel small RNA spike-in oligonucleotides enable absolute normalization of small RNA-Seq dataNext-generation sequencingSmall RNAsNormalization of high-throughput small RNA sequencing (sRNA-Seq) data is required to compare sRNA levels across different samples. Commonly used relative normalization approaches ...
血清和血浆中主要含有小RNAs,因此无需分开纯化小RNA和大RNA组分。 使用miRNeasy Serum/Plasma Spike-In Control进行标准化 使用miRNeasy Serum/Plasma Kit纯化miRNA时,加入QIAzol Lysis Reagent后,在加入氯仿分相前,向样本中加入miRNeasy Serum/Plasma Spike-In Control。real-time RT-PCR后,合成miRNA的CT值可对样本进...
We used a newly developed pool of 96 synthetic RNAs with various lengths, and GC content covering a 220 concentration range as spike-in controls to measure sensitivity, accuracy, and biases in RNA-seq experiments as well as to derive standard curves for quantifying the abundance of transcripts....
500 ng of total RNAs are converted into cDNA using cDNA synthesis kit (Takara). Syber green-based qPCR was performed using primer sets (Table S1) with 50 ng of cDNA from each sample in the QuanStudio-6 qPCR system (Applied Biosystems) with thermocycler condition: Stage1: 95 °...
the inoculum was removed, and the specimens were washed three times with PBS. Tissue cubes were maintained in DMEM/F12 medium supplemented with different concentrations of the indicated drugs for an additional 48 h. Then the tissues were harvested and the viral RNAs copies were measured by RT...
This airway tissue was produced from a different donor than that used in Fig. 3. The tissues were washed by DPBS to collect the secreted viruses every day from days 1 to 5. The total RNAs were isolated and amplified by RT–PCR. The ratio of D614 and G614 viruses after competition ...