Overall, the DNA aptamer developed in this study has the potential to bind to the SARS-CoV-2 spike protein and inhibit or block its interaction with ACE2. Thus, our DNA aptamers can be used as new biological tools for the prevention and diagnosis of SARS-CoV-2 infection....
DNA aptamer-linked sandwich structure enhanced SPRi sensor for rapid, sensitive, and quantitative detection of SARS-CoV-2 spike protein The harm and impact of the COVID-19 pandemic have highlighted the importance of fast, sensitive, and cost-effective virus detection methods. In this study,... ...
SARS-CoV-2 spike protein binds with angiotensin-convertingenzyme 2 (ACE2), a key prot... AH Williams,CG Zhan - 《Journal of Physical Chemistry B》 被引量: 0发表: 2021年 A new DNA aptamer which binds to SARS-CoV-2 spike protein and reduces pro-inflammatory response COVID-19 caused by...
in order to increase the feasibility of screening strategies, recombinant S1 protein was thrown to capture the entire SARS-CoV-2. In order to ensure the evolution efficiency of the affinity and specificity of aptamer candidates, we adopted
In the current study, a new approach has been developed to design of G-quadruplex DNA aptamer against SARS-CoV-2 spike protein. G-quadruplex DNA aptamers have G-rich sequences with the ability to form four-stranded structures that are crucial for ligand binding and biocatalysis [30]. However...
Toward addressing the unmet need of a reliable diagnostic test in the current work by utilizing the power of Systematic Evolution of Ligands by EXponential enrichment, a 44-mer G-quadruplex-forming DNA aptamer against spike trimer antigen of SARS-CoV-2 was identified. The lead aptamer candidate ...
Methods for the rapid detection of the presence of variants of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) that contain mutations in the Spike (S) protein gene in a
The receptor-binding domain (RBD) of spike protein from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is involved in the early stages of viral infection, is a promising target for aptamer selection. Currently, there are no effective approaches to prevent virus from ...
2.5. Protein expression and purification The DNA fragment encoding S-RBD (residues 319–541) was subcloned into the mammalian expression vector pTT5 with a C-terminal 6 × His tag. The high-quality plasmids were transfected into HEK293F cells by PEI (Polysciences, Warrington, PA, USA). Afte...
Those identified segments of DNA are then introduced into the bacteria to produce large amounts of protein fragments for development of ESV. This method dramatically shortens the time needed to produce a potential ESV against SARS-CoV2. Another approach is to develop a mRNA that stimulates the ...