Overall, the DNA aptamer developed in this study has the potential to bind to the SARS-CoV-2 spike protein and inhibit or block its interaction with ACE2. Thus, our DNA aptamers can be used as new biological tools for the prevention and diagnosis of SARS-CoV-2 infection....
in order to increase the feasibility of screening strategies, recombinant S1 protein was thrown to capture the entire SARS-CoV-2. In order to ensure the evolution efficiency of the affinity and specificity of aptamer candidates, we adopted
2.5. Protein expression and purification The DNA fragment encoding S-RBD (residues 319–541) was subcloned into the mammalian expression vector pTT5 with a C-terminal 6 × His tag. The high-quality plasmids were transfected into HEK293F cells by PEI (Polysciences, Warrington, PA, USA). Afte...
Those identified segments of DNA are then introduced into the bacteria to produce large amounts of protein fragments for development of ESV. This method dramatically shortens the time needed to produce a potential ESV against SARS-CoV2. Another approach is to develop a mRNA that stimulates the ...
Finally, the application of best performing aptamer candidate was demonstrated to detect the presence of spike trimer antigen in clinical specimens, nasopharyngeal swabs (NP, n = 232). SELEX-based identification of DNA aptamers against spike trimer protein of SARS-CoV-2 For aptamer selection, ...
We conducted a comparative study of 9 DNA aptamers for binding to several variants of the S-protein and pseudoviruses using the same testing methods, including dot-blot assays and enzyme-linked aptamer binding assays, to evaluate their affinity ranking and analytical utility. Moreover, the binding...
The receptor-binding domain (RBD) of spike protein from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is involved in the early stages of viral infection, is a promising target for aptamer selection. Currently, there are no effective approaches to prevent virus from ...
In this study, we developed an aptamer-based electrochemical biosensor to detect the SARS-CoV-2 spike protein, a viral antigen commonly utilized in diagnostic assays in a sandwich assay. To fabricate the electrochemical biosensor, a capture aptamer was immobilized onto a gold electrode. The target...
The aptaMIP exhibits an equilibrium dissociation constant of 1.61 nM toward its template which matches or exceeds published examples of imprinting of the spike protein. The work here demonstrates that "fixing" the aptamer within a polymeric scaffold increases its capability to selectivity recognize its...
Methods for the rapid detection of the presence of variants of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) that contain mutations in the Spike (S) protein gene in a