抗S1P抗体结合S1P涂层板或样品中的S1P。经过孵育和洗板后,将streptavidin-HRP(链霉亲和素-辣根过氧化物酶)添加到板中,结合所有结合在板上的抗s1p抗体(标记生物素)。在额外的孵育和洗板后,TMB底物被添加到板中,并且通过添加1N硫酸停止了比色反应。测定450nm处的吸光度,并与标准曲线进行对比,确定了样品中S1P的浓度...
ME Skaznikwikiel - 《Biology of Reproduction》 被引量: 79发表: 2006年 Sphingosine 1-phosphate signaling is involved in skeletal muscle regeneration Sphingosine 1-phosphate (S1P) is a bioactive lipid known to control cell growth that was recently shown to act as a trophic factor for skeletal ...
Abstract Two related lysosphingolipids, sphingosine 1-phosphate (S1P) and sphingosylphosphorylcholine (SPC) mediate diverse cellular responses through signals transduced by either activation of G-protein coupled receptors or possibly by acting intracellularly. Vascular responses to S1P and SPC measured both...
Figure 1. Cell viability (% of control) of preadipocyte SGBS cells (A), preadipocyte 3T3-L1 cells (B), differentiated adipocyte SGBS cells (C) and differentiated adipocyte 3T3-L1 cells (D) under sphingosine-1-phosphate (S1P) treatment (10–5000 nm) for 24 h, 48 h, and 72 h, respecti...