Protein regionalization on the spermatozoon surface was achieved by comparative analysis of the surface protein extracts from the entire spermatozoa and from periacrosomal sperm plasma membranes. Identification of several known proteins and of new proteins related to the process of epididymal maturation ...
Gamete recognition and binding are mediated by specific proteins on the surface of the sperm and egg. Identification and characterization of some of these proteins from several model systems, particularly mouse and sea urchin, have focused interest on the general properties and functions of gamete rec...
Protein Phosphatase 2A The discovery that protein phosphatases trigger retraction of fibers led to the identification of an endogenousPP2AinAscarissperm. This phosphatase is concentrated at the cell body–lamellipod junction where fiber complexes are taken apart. Purified PP2A from sperm can induce retrac...
The Identification of Mouse Sperm-Surface-Associated Proteins and Characterization of Their Ability to Act as Decapacitation Factors Nixon B.The identification of mouse sperm-surface-associated proteins and characterization of their ability to act as decapacitation factors.Biology of ... B Nixon,DA ...
Another center of attention is the physiological function of individual proteins, including studies of their cellular redox regulation and the identification of endogenous substrates. Together, these topics also constitute the main focus of this review, where we will limit ourselves to the mammalian syst...
et al. Towards a semen proteome of the dengue vector mosquito: protein identification and potential functions. PLoS Negl. Trop. Dis. 5, e989 (2011). Article CAS PubMed PubMed Central Google Scholar Boes, K. E. et al. Identification and characterization of seminal fluid proteins in the ...
Among these methods are the identification of Y-chromosome bearing spermatozoa by bi-functionalised gold nanoparticles and triplex hybridisation in vivo as well as new laser-controlled deflection system that replaces the deflection of spermatozoa in the electrostatic field. Additionally, as well as a ...
Knowledge of the sperm-specific protein that is required for the attachment of sperm to eggs during fertilization in mammals has led to the identification of the protein's receptor on the egg's plasma membrane. See Article p.483 Little is known about how
Identification of putative drug targets for human sperm-egg interaction defect using protein network approach Sperm-egg interaction defect is a significant cause of in-vitro fertilization failure for infertile cases. Numerous molecular interactions in the form of p......
2D-PAGE separation of sperm proteins and protein identification Five micrograms of each protein sample was selected for 2D-PAGE separation and mixed with 5 μg of the internal standard proteins, then rehydration buffer was added to a volume of 340 μL. The samples were loaded onto 18 cm Immo...