Glycosylphosphatidylinositol (GPI)-anchored proteins are attached to the lumenal side of membranes of the secretory and endocytic pathways by a glycolipid anchor. As they are not exposed to the cytoplasm, the mechanism of their sorting in the secretory/endocytic system does not fit the normal model...
Glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) are a diverse class of proteins that are anchored to the membranesolely via means of a post-translational lipid modification, the GPI-moiety. Since their discovery in the late 1970s, yearsof research have provided significant insight ...
Glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) are a diverse class of proteins that are anchored to the membranesolely via means of a post-translational lipid modification, the GPI-moiety. Since their discovery in the late 1970s, yearsof research have provided significant insight int...
Sorting GPI-anchored proteins. Nat Rev Mol Cell Biol 2004; 5:110-120. 62 Brown DA, Crise B, Rose JK. Mechanism of membrane anchoring affects polarized expression of two proteins in MDCK cells. Science 1989; 245:1499-1501. 63 Lisanti MP, Sargiacomo M, Graeve L, Saltiel AR, Rodri- gu...
Finally, using tethering mutants, we could measure GPI-anchored protein sorting in vivo and show that ypt1-3 and uso1-1 mutants are defective for sorting in vivo. Results Uso1p Is Required for Protein Sorting upon ER Exit Recently, we showed that GPI-anchored proteins were transported from ...
To understand the mechanism involved in the apical sorting of glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) we fused to the C-terminus of GFP the GPI-anchor-attachment signal of the folate receptor (FR) or of the prion protein (PrP), two native GPI-anchored proteins that are...
We studied the role of the association between glycosylphosphatidylinositol (GPI)-anchored proteins and glycosphingolipid (GSL) clusters in apical targeting using gD1-DAF, a GPI-anchored protein that is differentially sorted by three epithelial cell lines. Differently from MDCK cells, where both gD...
1992. Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface. Cell 68: 533–544. Brown MC, Besio-Moreno M, Bongarzone ER, Cohen PD, Soto EF, et al. 1993. Vesicular... SN Fewou,N Jackman,GV Meer,... - Springer US 被引...
Glycosyl-phosphatidylinositol (GPI)-anchored proteins (GPI-APs) are present at the surface of living cells in cholesterol dependent nanoscale clusters. The... R Chadda,MT Howes,SJ Plowman,... - 《Traffic》 被引量: 188发表: 2010年 The epithelial-specific adaptor AP1B mediates post-endocytic re...
Fluorescence-activated cell sorting (FACS) can be a useful method to studyGPIanchor expression, which can be affected in the GPI anchoring biogenesis defects as well as defects affecting the synthesis of the GPI anchor precursors. The most studied GPI-anchored proteins includeCD55, CD16...