Glycosylphosphatidylinositol (GPI)-anchored proteins are attached to the lumenal side of membranes of the secretory and endocytic pathways by a glycolipid anchor. As they are not exposed to the cytoplasm, the m
Proteins that were previously shown to be required for tethering and fusion of ER-derived vesicles with the Golgi compartment have been shown to have an additional function in GPI-anchored-protein sorting.GPI-anchored proteins are found in very small microdomains at the plasma membrane. They can ...
ER membrane protein p24 is involved in the sorting of GPI-anchored proteins by recruiting COPII components45. The effect ofsun3/4/5is distinct from the Arabidopsis loss-of-function mutant ofp24, in which the GPI-anchored protein AGP4 is retained in the ER46. The SUN3/4/5-POD1 complex...
Finally, using tethering mutants, we could measure GPI-anchored protein sorting in vivo and show that ypt1-3 and uso1-1 mutants are defective for sorting in vivo. Results Uso1p Is Required for Protein Sorting upon ER Exit Recently, we showed that GPI-anchored proteins were transported from ...
Interestingly, endogenous GPI-anchored proteins, which are mainly sorted to the AP surface in primary thyrocyte cultures [37] and in other epithelia [15], are preferentially delivered to the BL surface in FRT cells [38] indicating that the sorting machinery can vary considerably between different...
(GPI)-anchored placental alkaline phosphatase (PLAP) and two chimeric transmembrane proteins containing the PLAP ectodomain in stably transfected Madin-Darby canine kidney epithelial cells to determine whether different mechanisms might be used in apical sorting of GPI-anchored and transmembrane proteins. ...
hand, proteins anchored by glycophosphatidylinositol (GPI), or so-called GPI-anchored proteins, always parti- ttiioonnstointfhlueeLnocpehtahseepdauretittoiotnhiencghoefmpircoatleainffsinbietytwoef eGnPpIhtoastehsisispghiavseen11i0n. A thorough review of how lipid modifica- ref. 112. Among...
The GPI-anchor and protein sorting Glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) are a diverse class of proteins that are anchored to the membranesolely via means of a post-... S.,ChatterjeeS.,Mayor - 《Cellular & Molecular Life Sciences Cmls》 被引量: 375发表: 2001年 ...
The rhoptry of the malaria parasite Plasmodium falciparum is an unusual secretory organelle that is thought to be related to secretory lysosomes in higher eukaryotes. Rhoptries contain an extensive collection of proteins that participate in host cell inv
In contrast, the sorting machinery in both oligodendrocytes and MDCK cells recognizes targeting signals for MOG and PLP, and we propose that these oligodendroglial membrane proteins delineate cognate basolateral and apical domains, respectively.