Droplets were prepared via annealing in a buffer containing 10 mM Tris-HCl (pH 7.5 at 25 °C), 25 mM Mg2+ and 10 mM Na+. The arrested Pfu RPR droplets then underwent thermal cycling via temperature-controlled microscopy showing the subsequent relaxation of the Pfu RPR condensates...
Droplets were prepared via annealing in a buffer containing 10 mM Tris-HCl (pH 7.5 at 25 °C), 25 mM Mg2+ and 10 mM Na+. The arrested Pfu RPR droplets then underwent thermal cycling via temperature-controlled microscopy showing the subsequent relaxation of the Pfu RPR condensates...
trachomatis. The HSV-2 gD2 gene specific primer pair (gD2-F: 5′-TCAGCGAGGATAACCTGGGA-3′, gD2-R 5′-GGGAGAGCGTA CTTGCAGGA-3′ was used with a 69 °C annealing-extension temperature [64,65]. 4. Conclusions All chloride complexes (1, 3, 5, 7) as well as PTA complex 8 were ...
particle swarm optimisation, colony optimisation, cuckoo search, harmony search, bat-inspired algorithms, firefly algorithms, etc.; and (ii) non-nature metaheuristics, such as tabu search, variable neighbourhood search, iterated local search, iterated greedy, simulated annealing, greedy adaptive search ...
Hierarchical simulated annealing vs. a Gauss-Newton scheme applying analytical Jacobians for the solution of a source current distribution problem, ArtículoSummary: We consider the linear problem, where in a certain domain the magnetic field, generated by infinitely long conductors, whose locations as...
aUnless specified otherwise, all austenitic stainless steel materials shall be furnished in a solution annealed condition. Approved repairs, if any, shall be made prior to solution annealing 除非另外说明,所有奥氏体的不锈钢的材料在解答锻炼的情况将被装备。 批准的修理,若有的话,在解答焖火之前将被进行...
In the third phase, a modified Simulated Annealing is used to improve the solution given by the second phase. Computational results showed that TPA outperformed HDDE by Wang et al. (2010) and IG1 and IG2 by Ribas et al. (2011) in quality of solution. Chowdhury, Ghosh, and Sinha (...
This amount decreased to 42.5% as the annealing temperature increased to 250 °C (Fig. 5(e)), when the spec- t(ptor2au1stm.sh4iav%rtae)tos,ifeoamnandsblatltayehndeerdtraihesrawdstuaoHfsffifaacOisce2lonifgnitlhlvmyteondwxteiihcodrenienzaaelsadYenw2fnOoher3aetlnfheildeamn2a2n5t0,...
As an example, we present the solution of a simplified problem commonly occuring in laser-annealing studies of materials. Numerical tests which have been carried out are given consideration below. 展开 DOI: 10.1016/0010-4655(91)90035-J
Using simulated annealing, restrained molecular dynamics and energy minimization, the detailed structure of magainin 2 bound to DPC micelles was calculated and determined to form a well-defined helix across residues 4–20 (Fig. 1). Additionally, the peptide was curved with a kink centered around ...