Moreover, that mice expressing G85R SOD1 differ clinically (later onset plus rapid and synchronous motor neuron loss after onset) and pathologically (numerous astroglial inclusions and absence of vacuoles) from G93A and G37R SOD1 mice may be explained by differences 1) in the toxic properties...
SOD1-ALS and TDP43-ALS were generated in biological triplicates (3 independent differentiations side by side) and 1 µg of total RNA was isolated and validated as previously described and was used for selection of Poly(A) plus RNA and library preparation was done after oligo (dT) selectio...
The addition of rotenone plus antimycin C induced a reduction in OCR values to approximately 10 pmol/min/2 × 105 cells in control and ALS lymphoblasts (Figure 1C). The approximately 10% difference in OCR values between oligomycin and rotenone exposure in the three groups...
(d) Bar graph depicting DCF- DA-detected ROS production in NSC-34 motor neurons exposed to L-BMAA (300 μM/48 h) or L-BMAA plus SOD1 (400 ng/ml). Data are expressed as mean ± S.E. of three different experiments. *Po0.05 versus control and 400 ng/ml SOD1+L-BMAA counteracted...
Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 Catalog # 31460 Invitrogen Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Catalog # A21246 Invitrogen F(ab')2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ ...
(pH 7.6) containing: 15 mM Tris–HCl, 1 mM dithiothreitol, 250 mM sucrose, 1 mM MgCl2, 2.5 mM EDTA, 1 mM EGTA, 250 mM Na3VO4, 25 mM NaF, 2 mM sodium pyrophosphate, 0.5 mM phenylmethylsulfonyl fluoride, plus 1 μg/ml pepstatin A, 5 μg/ml leupeptin, and 2.5 μg/ml aprop...
Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 647 Catalog # 31460 Invitrogen Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Catalog # A11009 Invitrogen Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 532篇...
Pictures were taken with a Zeiss Mirax digital scan- ning microscope, a Zeiss Axiovert 200 or Axioskop 2plus microscope, post processing was carried out using adobe photoshop. Paraffin-embedded tissue blot Pieces of PVDF membrane (20 mm x 50 mm Fluoro- bind, Serva, Heidelberg, Germany) were...
The protein samples (20 µg protein) were separated on Bolt 4–12% Bis-Tris Plus gels (Thermo Fisher Scientific, Waltham, MA, USA) and transferred to polyvinyli- dene difluoride membranes (Bio-Rad Laboratories, Hercules, CA, USA). The membranes were incubated with primary and matched ...
The cDNA template was amplified on a StepOnePlus Real-Time PCR Thermocycler (Life Technologies) with SYBR Green Mastermix (Applied Biosystems) or with qPCRBIO SyGreen Blue Mix Hi-ROX (PCR Biosystems). The same amplification method was used for similar experiments. qPCR data was analysed using ...