NSC34 cells were incubated in complete DMEM medium in the absence or presence of A77 1726 (200 μM) minus or plus uridine (200 μM) for 16 h. Cell lysates were analyzed for LC3-II lipidation and actin expression by western blot. The expression levels were analyzed by quantification...
Dcoument S1. Figures S1–S7 and Tables S1–S4 Table S1. Long-lived proteins Table S2. SOD1 +/A4V MN persisting proteins Table S3. VCP-interacting proteins Table S4. Samples analyzed by LC-MS/MS and deposited at MassIVE database Document S2. Article plus supplemental informationVolume...
The protein samples (20 µg protein) were separated on Bolt 4–12% Bis-Tris Plus gels (Thermo Fisher Scientific, Waltham, MA, USA) and transferred to polyvinyli- dene difluoride membranes (Bio-Rad Laboratories, Hercules, CA, USA). The membranes were incubated with primary and matched ...
期待期待，感觉应该会很好玩，越来越觉得plus有意思了。