Current single-cell RNA sequencing (scRNA-seq) methods with high cellular throughputs sacrifice full-transcript coverage and often sensitivity. Here we describe Smart-seq3xpress, which miniaturizes and streamlines the Smart-seq3 protocol to substantially reduce reagent use and increase cellular throughpu...
Separated per protocol (Smart-seq2 and Smart-seq3) and experiment (HEK293FT, Mouse Fibroblasts, HCA cells). (d) Mapping statistics for 5’UMI-containing read pairs in Smart-seq3. Percentage of unmapped read pairs, and read pairs that aligned to exonic, intronic and intergenic regions. ...
Single-cell TCR sequencingWe developed an automated high-throughput Smart-seq3 (HT Smart-seq3) workflow that integrates best practices and an optimized protocol to enhance efficiency, scalability, and method reproducibility. This workflow consistently produces high-quality data with high cell capture ...