and automated RNA extraction of small volumes of blood using the Tempus Spin RNA isolation kit and the MagMAX for Stabilized Blood RNA Isolation kit , respectively; and (ii) assessed the effect of TURBO DNA Free treatment on the transcriptomic data of RNA isolated from small volumes of blood....
RNA isolation Per 2.5 ml whole blood we isolated about the same amount (mean) of total RNA from seminoma patients without metastasis (7.7 μg, +/-1.9), lymphogen metastasis (4.9 μg, +/-2.0 μg) and occult metastasis (6.8 μg, +/-2.4, Table 1). The RNA integrity number (RIN) ...
Identification of MenSC-derived small EVs and their therapeutic potentials for tissue repair in various diseases. Small EVs from MenSCs consist of regulatory proteins, RNAs, and DNAs, lipids, and siginaling peptides promoting regenerative repair of wounded cells and tissues. MenSC-derived small EV...
Breast cancer is the most common cancer affecting women across the world. Tumor endothelial cells (TECs) and malignant cells are the major constituents of the tumor microenvironment (TME), but their origin and role in shaping disease initiation, progress
We further show, using unique molecular identifiers, that stochastic capture of low input RNA rather than PCR amplification influences the biased quantitation of intermediately and lowly expressed microRNAs. Our improved method allows the processing of tens to hundreds of samples simultaneously while ...
Approximately 200 μL of plasma was obtained from each mouse and was equally divided into two samples, one (~100 μL) for direct isolation of total RNA and quantification of total siRNA levels, and the other for purification of exosomes. Plasma exosomes were isolated using the Total ...
Protein and small non-coding RNA-enriched extracellular vesicles are released by the pathogenic blood fluke Schistosoma mansoni. J Extracell Vesicles 2015; 4:28665; PMID:26443722; http://dx.doi.org/10.3402/jev. v4.28665NOWACKI F C, SWAIN M T, KLYCHNIKOV O I, et al. Protein and small ...
Cell pelleting, lysis and RNA isolation was performed as described above (Small RNA SLAMseq in Drosophila S2 cells). 50 μg total RNA was fragmented in 20 μl 1x RNA Fragmentation buffer for 1 min at 94°C (NEBNext® Magnesium RNA Fragmentation Module), resulting in ∼1 kb fragments...
In conclusion, the dual-targeting M.RGD@Cr-CTS-siYTHDF1 NPs, integrating dual-targeting capabilities with photothermal therapy (PTT) and RNA interference, offer a promising approach for molecular targeted cancer immunotherapy with potential for clinical application. Graphical Abstract...
Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Curr. Protoc. Cell Biol. Chapter 3, Unit 3.22, (2006). PubMed Google Scholar Peltier, H. J. & Latham, G. J. Normalization of microRNA expression levels in quantitative RT–PCR assays: ...