mRNA and small RNA gene expression changes in peripheral blood to detect internal Ra-223 exposureWe evaluated whole blood samples from five patients with castration-resistant prostate cancer and multiple bone metastases (without visceral or nodal involvement), who underwent treatment with the alpha ...
Small Cytoplasmic RNA In subject area: Pharmacology, Toxicology and Pharmaceutical Science About this pageSet alert Also in subject areas: Biochemistry, Genetics and Molecular Biology Medicine and Dentistry NeuroscienceShow moreDiscover other topics
RNA adopts 3D structures that confer varied functional roles in human biology and dysfunction in disease. Approaches to therapeutically target RNA structures with small molecules are being actively pursued, aided by key advances in the field including the development of computational tools that predict ...
Tosar, J. P. et al. Assessment of small RNA sorting into different extracellular fractions revealed by high-throughput sequencing of breast cell lines.Nucleic Acids Res.43 ArticleCASGoogle Scholar Hunter, M. P. et al. Detection of microRNA expression in human peripheral blood microvesicles.PLoS ...
Figure 1. The Classification and Biogenesis of tsRNA There are more than 6 types of tsRNAs. Each type of tsRNA is processed from mature tRNA or pre-tRNA and has a distinct biogenesis pathway generated by different ribonucleases. All of the involved processing enzymes are indicated; the major...
2.2. Serum RNA isolation Serum and tissue samples were collected from all patients prior to starting immunotherapy and during treatment. Sera RNA was extracted using the miRNeasy Mini kit (Qiagen) following the manufacturer's protocol. Five hundred microliters of plasma was mixed with 2.5-volume of...
All RNA-sequencing data generated in this study have been deposited as a superseries at the NCBI Gene Expression Omnibus with the accession codeGSE174630. The crystal structure data for SND1–C26-A2 (PDB ID:7KNW) and SND1–C26-A6 (PDB ID:7KNX) have been deposited at the Protein Data...
DNA isolation Genomic DNA was isolated from the blood samples using Qiagen DNA Isolation kit (Catalogue No. 51206). The quantity and quality control analysis of genomic DNA was performed by carrying out UV spectrophotometer (Eppendorf Biospectrometer®, Hamburg Germany) analysis and Gel electrophoresi...
Total RNA was extracted by Tripure Isolation Reagent (Roche, Basel Switzerland). For evaluation of mRNA expression, HiScript II Q RT SuperMix Kit (Vazyme, Nanjing, China) was used to reverse-transcribe RNA into cDNA. Expression inflammatory gene levels (Il1b, Tnf-α, Il6, Nos 2, Mrc1, ...
After a wash step, the small RNA is eluted. Also provided is a method for the isolation of large RNA, by eluting the large RNA from the first mineral support. In addition, total protein is present in the filtrate and can be isolated by a conventional method. WO9506652 refers to ...