RNA/RNA interactionstranslationtranscriptionIn recent years, small regulatory RNAs have been discovered at a staggering rate both in prokaryotes and eukaryotes. By now it is clear that post-transcriptional regulation of gene expression mediated by such RNAs is the rule rather than—as previously ...
Raya Faigenbaum-RommAsaf PeerNiv ReissHanah MargalitNat ProtocNature ProtocolsMelamed S, Faigenbaum-Romm R, Peer A, Reiss N, Shechter O, Bar A, et al. Mapping the small RNA interactome in bacteria using RIL-seq. Nat Protoc. 2017;13:1-33. https://doi.org/10.1038/nprot.2017.115....
Competing endogenous RNAs: a target-centric view of small RNA regulation in bacteria. Nat Rev Microbiol. 2016;14(12): 775-84.Bossi, L. & Figueroa-Bossi, N. Competing endogenous RNAs: a target-centric view of small RNA regulation in bacteria. Nat. Rev. Microbiol. 14, 775-784 (2016)....
these small but powerful molecules primarily because the originalRNAisolation protocols, and later RNA isolation kits, were not designed to capture such small transcripts. It is nowevident that these small transcripts are of supreme regulatory importance, and kits for the isolation of small RNAs ...
One mechanism that assists bacteria to cope with acute environmental changes (hereafter termed stress) involves regulatory small RNAs (sRNA), which are present in a wide array of bacterial species. sRNAs contribute mainly to posttranscriptional control (RNA degradation or translation) by pairing with...
Genomic DNA is a concern especially for bacterial RNA sequencing as the cDNAs corresponding to the contaminating genomic DNA will result in inaccurate representation of the expressed transcripts [17]. Since DNA is usually co-extracted during the RNA isolation procedures, evaluating the presence of cont...
aeruginosaresponds to environmental cues and accordingly switches infection lifestyles within the mammalian host. In this study, we addressed this gap in knowledge by leveragingP. aeruginosatranscriptomes acquired from human-derived samples to discover and mechanistically characterize a new small RNA,...
the RiboPure™-Blood Kit (Cat #AM1928) can be used to isolate total RNA that includes the small RNA fraction from RNA later® Solution-treated human blood samples. Using this modified protocol, total RNA that includes miRNA,...
Recent discoveries on bacterial transcriptomes gave evidence that small RNAs (sRNAs) have important regulatory roles in prokaryotic cells. Modern high-throughput sequencing approaches (RNA-Seq) enable the most detailed view on transcriptomes offering an
Small RNAs (sRNAs) within 15-30 nt such as miRNA, tsRNA, srRNA with 3’-OH have been identified. However, whether these sRNAs are the major 15-30 nt sRNAs is still unknown. Here we show about 90% mammalian sRNAs within 15-30 nt end with 2’,3’-cyclic ph