RNA/RNA interactionstranslationtranscriptionIn recent years, small regulatory RNAs have been discovered at a staggering rate both in prokaryotes and eukaryotes. By now it is clear that post-transcriptional regulation of gene expression mediated by such RNAs is the rule rather than—as previously ...
细菌非编码小RNA(small non-coding RNA, sRNA)是一类长度在50~500个核苷酸, 不编码蛋白质的RNA。迄今, 在各种细菌中共发现超过150多种sRNA。它们通过碱基配对识别靶标mRNA, 在转录后水平调节基因的表达, 是细菌代谢、毒力和适应环境压力的重要调节因子。细菌sRNA的研究技术主要有基于生物信息学的计算机预测法和基于...
Conducting further analyses, the Bochum-based researchers found out that small RNA manipulates numerous processes, those including biosynthesis of the bacterial cell wall. If the bacteria lacked this molecule, however, they formed more plant tumours than the natural strain. In nature, as the researche...
Small RNAs (sRNAs) within 15-30 nt such as miRNA, tsRNA, srRNA with 3’-OH have been identified. However, whether these sRNAs are the major 15-30 nt sRNAs is still unknown. Here we show about 90% mammalian sRNAs within 15-30 nt end with 2’,3’-cyclic ph
micF RNA binds to the 5’ end of ompF mRNA and to a protein from Escherichia coli. Biochemistry 29, 9249–9256 (1990). CAS PubMed Google Scholar Delihas, N. Discovery and characterization of the first non-coding RNA that regulates gene expression, micF RNA: a historical perspective. ...
One mechanism that assists bacteria to cope with acute environmental changes (hereafter termed stress) involves regulatory small RNAs (sRNA), which are present in a wide array of bacterial species. sRNAs contribute mainly to posttranscriptional control (RNA degradation or translation) by pairing with...
Approximately 200 μL of plasma was obtained from each mouse and was equally divided into two samples, one (~100 μL) for direct isolation of total RNA and quantification of total siRNA levels, and the other for purification of exosomes. Plasma exosomes were isolated using the Total ...
Improved method for the isolation of RNA from plant tissues. This method can be adapted to a small-scale version by the use of 1.5-ml tubes, allowing rapid isolation of RNA from a larger number of samples. ... J Logemann,J Schell,L Willmitzer - 《Analytical Biochemistry》...
On the other hand, DNA repair (HMGA1), RNA editing or degradation (AARSD1; ZFN326; GTPBP1) and a NF-κB regulator (UBFD1) were found to be depleted (Supplementary Fig. S4a,b; Supplementary Tables S2–S5 online). Upon challenges of persistent mtROS production and potential mitochondrial ...
For a global view of small RNAs expressed in Chinese cabbage, we chose to collect material from the above ground portions of HT and MT seedlings and sequenced RNA fractions of 9 to 36 nucleotides (nt), using Illumina Genome Analyzer and mirVana™ miRNA Isolation Kit (Ambion, Inc). Two re...