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Age-associated loss of muscle mass and function and subsequent mobility decline define poor health outcomes, reduced quality of life, and mortality risk. T
E, like D, but showing co-modified proteins annotated to the biological process of DNA repair. F, Co-modified proteins annotated to the biological process of the cell cycle, including targets involved in the more specific process of mitotic cell cycle (orange outline). Larger shape size ...
software was used to perform raw MS/MS data format trans- formation to msm-files for peptide sequence search. Mascot search engine against the Swisssprot Homo_sapiens database were performed with the following parameters were allowed: tryptic peptides with 0 to 2 missed cleav- age sites; the ...
RAW2MSM (version 1.1.) software was used to perform raw MS/MS data format transformation to msm-files for peptide sequence search. Mascot search engine against the Swisssprot Homo_sapiens database were performed with the following parameters were allowed: tryptic peptides with 0 to 2 missed cle...
After 21–24 h of incubation at 37 °C with 5% CO2, the culture medium was removed, the cells were washed with 100 µL/well of 1× PBS, and 25 µL of 1× Passive Lysis Buffer (5× Passive Lysis Buffer (Promega, Madison, WI, USA) diluted to 1/5) was added to each well....
The gel slices were digested using 600 ng of trypsin (Promega, Madison, WI, USA) at 37 °C to build the spectral library. The trypsin digestion was stopped with 10% trifluoroacetic acid (TFA), and the supernatant was removed. Then, the library gel slices were dehydrated with pure ...
Since 30% of patients with advanced empyema have contraindications to surgical treatment, novel, low-dose, pharmacological treatments are needed. A Streptococcus pneumoniae-induced rabbit model of chronic empyema recapitulates the progression, loculation, fibrotic repair, and pleural thickening of human ...
hPBMCs (1 × 105) were plated in a 96 well plate and treated with either AVR-48 (0.1, 1.0, 10, 100 and 1000 µM), or BTAVR-48 (1, 10 and 100 µM) and incubated for 48 h. The cells were treated with MTT and resolving reagents (Promega, Madison, WI, USA), incubated fo...