Age-associated loss of muscle mass and function and subsequent mobility decline define poor health outcomes, reduced quality of life, and mortality risk. T
Brautigan contribution: “Horse Race”^, “The Wheel”^, “Karma Repair Kit: Items 1-4” [previously published in All Watched Over by Machines of Loving Grace (Barber 22)] [^previously published in collected in The Octopus Frontier (Barber 12)] (Barber 39) 48. MADISON KALEIDOSCOPE, Vol....
Among the five clusters, the two largest net- works were connected to two different functions: "DNA repair" and "rRNA metabolic processes". Both functions are related to chemotherapy, and some of these nodes have been reported to be cancer biomarkers. Burger et al. observed that inhibition of...
sequencing-grade trypsin was purchased from Promega (Madison, WI, USA). Ammonium persulfate (APS) and N,N,N′,N′-tetramethylenediamine (TEMED) were purchased from Amersham Pharmacia (Piscataway, NJ, USA). Polypropylene frits disk was purchased from Agilent (Wilmington, DE, USA). Ni-NTA silic...
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The bmp2b, bmp1a, tll1, and chrd mRNAs were generated from pCS2 + kzbmp2, pCS2 + bmp1a [11], pCS2 + tll1 [52], and pCS2 + chrd-myc [48] by restriction digestion with NotI and AmpliCap SP6 High Yield Message Maker Kit (CellScript, Madison, WI, USA). The synthesized capped...
The gel slices were digested using 600 ng of trypsin (Promega, Madison, WI, USA) at 37 °C to build the spectral library. The trypsin digestion was stopped with 10% trifluoroacetic acid (TFA), and the supernatant was removed. Then, the library gel slices were dehydrated with pure ...
Coombs, G.S.; Bergstrom, R.C.; Madison, E.L.; Corey, D.R. Directing sequence-specific proteolysis to new targets. The influence of loop size and target sequence on selective proteolysis by tissue- type plasminogen activator and urokinase-type plasminogen activator. J. Biol. Chem. 1998, 27...
hPBMCs (1 × 105) were plated in a 96 well plate and treated with either AVR-48 (0.1, 1.0, 10, 100 and 1000 µM), or BTAVR-48 (1, 10 and 100 µM) and incubated for 48 h. The cells were treated with MTT and resolving reagents (Promega, Madison, WI, USA), incubated fo...
Eight of the remaining 16 models had errors that we were unable to repair and eight had forms unsuited to prediction. Therefore, we excluded these 16 models. We also excluded models lacking goodness of fit information, models with adjusted R2 < 0.25, and sources older than 25 years. Among ...