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SLC7A11 的一抗 (12691 (Cell Signaling Technology)、ab175186 (Abcam) 和 26864-1-AP (Proteintech)、Flag 标签 (14793; Cell Signaling Technology)、ACSL4 (ab155282; Abcam)、链霉亲和素 (3419; Cell Signaling Technology)、GPX4(ab125066;Abcam)、LAMP2(66301-1-Ig;Proteintech)、Anti-4Hydroxynonenal(ab...
To investigate the detail regulation of STAT6 on ferroptosis, the differentiated THP-1 cells were exposed with CS for 24 h and the cell culture medium was harvested to further treat HBE cells (Fig.3B). CS medium treatment upregulated the expression of PTGS-2, which was further increased b...
(pH 6.0) in microwave for 30min, pre-diluted primary antibodies were incubated overnight at 4 °C [anti-4-HNE (1:100, MAB3249, R&D Systems), anti-Ki-67 (1:200, Abcam, ab16667), anti-SP1(1:600, 9389S, Cell Signaling Technology) and anti-SLC7A11(1:50, NB300–318SS, Novus)]...
(Cell Signaling Technology, 5832S, 1:1000 dilution), ATF-4 (D4B8) rabbit mAb (Cell Signaling Technology, 11815, 1:1000 dilution), NRF2 (D1Z9C) XP® rabbit mAb (Cell Signaling Technology, 12721, 1:1000 dilution), and α-tubulin antibody (Cell Signaling Technology, 2144, 1:5000 ...
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抗体均购自Cell signalingtechnology公司 Rabbit SLC7A11一siRNA一1,2,3。转染时,先将100pmol的待转染 (SLC7A11:SC一98552;13一actin:4970L;antiIgG: 7074S)。 质粒和5 万方数据 塑岱旦主瘤匿堂垫!曼生!旦 箜箜鲞笠兰翅 丛Q望垦垦盟Q盟垦Q坠Q堡!:』!翌:垫!三:yQ坠垫:盟Q:Q2 ·151· 一MEM@I...
Primary antibodies used in this study are as follows: PDIA4 (14712-1-AP, Proteintech), Caspase-3 (9664S, Cell Signaling Technology), Necroptosis antibody sampler kit (98110T, Cell Signaling Technology), LC3 (M152-3, MBL Life Science), SQSTM1 (ab56416, Abcam), ATG5 (ab108327, Abcam)...
Antibodies against xCT (98051 s, 1:1000), phospho-S6 (2211 s, 1:1000), S6 (2217 s, 1:1000), phospho-AMPKα (50081 s, 1:1000) and AMPKα (5831 s, 1:1000) were from Cell Signaling Technology (Danvers, MA, USA). HIF-1α antibody (nb100-105, 1:1000) was from (Novus Biolog...
Immunohistochemical staining for xCT protein using anti-xCT (#12691, Cell Signaling Technology, Danvers, MA) and scoring was conducted as previously described [41]. Briefly, the final staining score was calculated by multiplying the staining distribution score (no staining = 0; 0.1, staining ...