The RNA fragments were incubated with anti-m6A antibody (Sigma-Aldrich), followed by immunoprecipitation with protein-A beads (Invitrogen) and protein-G beads (Invitrogen). The library was sequenced on the NovaSeq high-throughput platform by PE150 sequencing mode. The raw sequence archives of m6A...
Zebrafish ZIP9 antigen peptide and antibody synthesis and purification were performed by GenScript (Piscataway, NJ). A peptide of the amino acid sequence HSHSPGGSAGKGLS was synthesized with a cysteine residue on the N-terminus to facilitate conjugation of keyhole limpet hemocyanin (KLH) and used f...