将蛋白样品与 5×上样缓冲液混合,100 ℃处理 5 min.电泳后转膜至 PVDF 膜,5%脱脂奶粉室温封 闭 2 h,加入 NR3C1,p53,SLC7A11 一抗(均按 1: 500 稀释),4 ℃孵育过夜,TBST 洗涤 4 次,加入相 应二抗(1:1 000),37 ℃孵育 2 h,TBST 洗涤 4 次, 滴加 ECL 化学发光液显影,凝胶成像系统进行分析, ...
Rabbit monoclonal anti-SLC7A11 Cell Signaling Technology Cat# 12691S; RRID:AB_2687474 Rabbit monoclonal anti-FTL Abcam Cat# ab109373; RRID:AB_10862715 Rabbit monoclonal anti-FTH1 Cell Signaling Technology Cat# 4393S; RRID:AB_11217441 Rabbit monoclonal anti-GPX4 Abcam Cat# ab125066; RRID:AB_109...