This method has successfully identified the cell type-specific transcriptome in three different tissues: endothelial cells in brain, epithelial cells in intestine, and adipocytes in white adipose tissue. Since this method does not require isolation of cells or RNA prior to the transcriptomic analys...
SLAMseq unites the patented 4-thiouridine (S4U) labeling technology with a streamlined workflow in the only commercially available, high-quality kit and is the method of choice to determine treatment effects on RNA synthesis or RNA turnover in cell culture experiments. ...
Analysis of cell-type-specific transcriptomes is vital for understanding the biology of tissues and organs in the context of multicellular organisms. In this Protocol Extension, we combine a previously developed cell-type-specific metabolic RNA labeling method (thiouracil (TU) tagging) and a pipeline...