The F-box protein UNUSUAL FLORAL ORGANS (UFO) is important for normal meristem identity and floral organ development [17–19]. UFO can interact with LEAFY genetically to activate AP3 expression [20–22]. The Arabidopsis homolog of the yeast and human SKP1 genes, the ARABIDOPSIS SKP1-LIKE1 ...
because the starting protein amount was about 20 % of the others to test whether a smaller amount of input protein extract could lead to different efficiency of protein detection. The test result did not show a huge difference in the detection efficiency when the amount of starting material was...
Most of the known ubiquitin ligase substrates were identified by protein-protein interaction methods, usually when the F-box protein has a known function [10,32–34]. Recently, mass spectrometry (MS)-based proteomics approaches have been increasingly applied in various areas including differential gen...
Protein degradation and turnover under various environmental stresses is basically regulated by ubiquitin-proteasome system (UPS), of which SKP1 is a very essential component. Isolation and cloning of an identified potential stress responsive candidate gene SKP1, was successfully done for the first tim...