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For staining in human samples, the slides were incubated with primary antibodies against Skp2 (Invitrogen, 1:100), mH2A1.1 (Cell signaling, 1:50), mH2A1.2 (1:100), CDK8 (Santa Cruz, 1:100), polyclonal cleaved caspase-3 (Cell Signaling, 1:100) and Ki-67 antibody (Epitomics, 1:160...
The antibody recognizing pSKP2 (S72) was a kind gift from Cell Signaling Technology®. Subsequently, blots were washed and probed with the respective horseradish peroxidase- or fluorophore-conjugated secondary antibody for 1 h at room temperature. The immuno-reactive bands were visualized either ...
2 μl of anti-HA antibody was added to each sample and incubated for 1 h. 20 μl of Protein A/G beads (Santa Cruz) were subsequently added to each sample and incubated for 1 h at 4 °C. Protein complexes on the beads were collected by brief centrifugation and analyzed by 12% SDS...
The antibodies used were anti-actin (goat polyclonal, sc-1616), MYC (sc-764), SKP2 (sc-7164), p27 (sc-528), α-tubulin (sc-5546) (all rabbit polyclonals from Santa Cruz Biotechnology), p27 monoclonal antibody (K-25020; Transduction Labs), and Thr(P)-187-p27 (rabbit polyclonal,...
Mcl-1 (sc-819) for immunoprecipitate was from Santa Cruz (Dallas, TX). Secondary antibodies, including anti-rabbit IgG HRP (#7074) and anti-mouse IgG HRP (#7076), were purchased from Cell Signaling Technology. Antibody conjugates were visualized by chemiluminescence (ECL; cat#34076, Thermo ...
sigma aldrich(西格玛) | Invitrogen(英骏) | Millipore | Abcam | CST | Roche | peprotech | prospec | pierce | BD Pharmingen | BD Biocoat | R&D | BioVision | Vector | Enzo life | eBioscience | ABR | Amresco | Cayman Chemical | 脂蛋白 | Merck | Qiagen(凯杰) | Santa cruz(三塔抗体...
sigma aldrich(西格玛) | Invitrogen(英骏) | Millipore | Abcam | CST | Roche | peprotech | prospec | pierce | BD Pharmingen | BD Biocoat | R&D | BioVision | Vector | Enzo life | eBioscience | ABR | Amresco | Cayman Chemical | 脂蛋白 | Merck | Qiagen(凯杰) | Santa cruz(三塔抗体...
(R&D Systems) | seven hills bioreagents | zytovision | zyagen Laboratories | ZeptoMetrix | WSU Monoclonal Antibody Center | VMRD | VitaNavi | Virusys | Usbiological | UBPBIO | tulip biolabs | TriLink BioTechnologies | TRC | Ted Pella | swant | steraloids | ST john's laboratory | Sphero...
sigma aldrich(西格玛) | Invitrogen(英骏) | Millipore | Abcam | CST | Roche | peprotech | prospec | pierce | BD Pharmingen | BD Biocoat | R&D | BioVision | Vector | Enzo life | eBioscience | ABR | Amresco | Cayman Chemical | 脂蛋白 | Merck | Qiagen(凯杰) | Santa cruz(三塔抗体...