Mice were perfused with 4% paraformaldehyde under anesthesia. The brain tissues were cut into 20 μm-thick sections after 30% DEPC-sucrose dehydration at 4 °C. Primary antibodies against AKAP150 (Santa Cruz, Cat# sc-377055, 1:200), phospho-TRPV1 (Ser502) (Affinity Biosciences, Cat#...