我们 通过绿色荧光蛋白追踪,定量 PCR 与热激实验等方法验证了 SKN-1 通过抑制 DAF-16 的入核从而影响线虫抗热性的猜想, 通过文献阅读了解到SKN-1 与线虫 卵黄蛋白原合成相关 VITs (vit-1,vit-2 ,vit-3 ,vit-4 ,vit-5,vit-6)基因之间的联 系,并通过油红染色,热激实验与GFP 荧光观察等方法验证SKN-1...
APE could regulate SKN-1, which was evident from qPCR analysis and the fluorescence of skn-1 :GFP transgenic strain. Further qPCR analysis of candidate regulatory genes exhibited antioxidant mechanisms induced by APE. CONCLUSION: APE was observed to induce anti-aging efficacy via SKN-1 and DAF-...
In C. elegans research, transcriptional activation of glutathione S-transferase 4 (gst-4) is often used as a read-out for SKN-1 activity. While many heed an assumed non-exclusivity of the GFP reporter signal driven by the gst-4 promoter to SKN-1, this is also often ignored. We here ...
elegans. Mitophagy in nematodes was measured using a mito- phagy reporter strain N2;Ex(pmyo-3::dsred::lgg-1;pdct-1::dct-1::gfp). Nematodes were exposed to tomatidine at L4 stage, followed by imaging of muscle cells at adult D4. A total of 15~20 nematodes/group were imaged ...
Additionally, D3 treatment did not alter the subcellular localization of a DAF-16::GFP fusion protein (TJ356 strain; data not shown). These data suggest that the effect of D3 feeding on lifespan extension is independent of DAF-16. In addition, we found that vitamin-D3-induced lifespan ...
(Phsp-6::GFP), endoplasmic reticulum unfolded protein response (Phsp-4::GFP), the SKN-1 mediated oxidative stress response (Pgst-4::GFP, Pgcs-1::GFP), hypoxia response (Pnhr-57::GFP), and the DAF-16 mediated stress response... CE Schaar 被引量: 0发表: 2016年 ...
It has been reported that GFP driven from the promoter region of mpk-1b is expressed in the pharyngeal-intestinal valve and nervous system (34). To identify the tissues in which mpk-1a is expressed, we constructed Pmpk-1a::Venus, in which the 4.2-kbp upstream region of mpk-1a is ...
elegans. Finally, we investigated effect of various OSCs from garlic and their analogs on induction of a gst-4p::GFP transgene, an indicator of SKN-1 activity. Results and Discussion SAC and SAMC extend C. elegans lifespan under normal conditions We first evaluated whether SAC and SAMC (...
To uncover regulators of the SKN-1/NRF2 homeostatic stress response network, a forward genetic screen with ethyl methanesulfonate 3363 (EMS) was performed on a gst-4p::GFP reporter strain, as a surrogate for SKN-1 activation (Fig. 1A) [9, 20]. From this screen, several classes of ...
Additionally, we find that the rpn-10 mutant also shows enhanced activity of the autophagy-lysosome pathway as evidenced by increased expression of the multiple autophagy genes including atg-16.2, lgg-1, and bec-1, and also by an increase in GFP::LGG-1 puncta. Consistent with a critical ...