我们 通过绿色荧光蛋白追踪,定量 PCR 与热激实验等方法验证了 SKN-1 通过抑制 DAF-16 的入核从而影响线虫抗热性的猜想, 通过文献阅读了解到SKN-1 与线虫 卵黄蛋白原合成相关 VITs (vit-1,vit-2 ,vit-3 ,vit-4 ,vit-5,vit-6)基因之间的联 系,并通过油红染色,热激实验与GFP 荧光观察等方法验证SKN-1...
APE could regulate SKN-1, which was evident from qPCR analysis and the fluorescence of skn-1 :GFP transgenic strain. Further qPCR analysis of candidate regulatory genes exhibited antioxidant mechanisms induced by APE. CONCLUSION: APE was observed to induce anti-aging efficacy via SKN-1 and DAF-...
In C. elegans research, transcriptional activation of glutathione S-transferase 4 (gst-4) is often used as a read-out for SKN-1 activity. While many heed an assumed non-exclusivity of the GFP reporter signal driven by the gst-4 promoter to SKN-1, this is also often ignored. We here ...
Additionally, D3 treatment did not alter the subcellular localization of a DAF-16::GFP fusion protein (TJ356 strain; data not shown). These data suggest that the effect of D3 feeding on lifespan extension is independent of DAF-16. In addition, we found that vitamin-D3-induced lifespan ...
(Phsp-6::GFP), endoplasmic reticulum unfolded protein response (Phsp-4::GFP), the SKN-1 mediated oxidative stress response (Pgst-4::GFP, Pgcs-1::GFP), hypoxia response (Pnhr-57::GFP), and the DAF-16 mediated stress response... CE Schaar 被引量: 0发表: 2016年 ...
参考价 ¥1 Y1-GFP细胞 Y1-GFP肾上腺皮质细胞 参考价 ¥1 SNU449细胞(SNU449肝癌细胞库) 参考价 ¥1产品分类品牌分类 传代细胞 HA细胞 Daudi细胞 Dami细胞 Calu-3细胞 CaSki细胞 BT474细胞 AGS细胞 ACHN细胞 A875细胞 A549细胞 A498细胞 细胞库 小鼠细胞 全部产品列表 产品简介 SKNSH细胞 人神经...
1). There was no difference observed between wild-type and osm-11. Moreover, the specificity and effectiveness of skn-1(RNAi) was confirmed by visualization of SKN-1 fused to GFP (Supplementary Fig. 1). In addition, RNAi against an independent essential gene, let-413(RNAi), was fully ...
The mitophagy-screening strain N2;Ex(pmyo- 3::dsred::lgg-1;pdct-1::dct-1::gfp) was used for mitophagy detection. A set of representative images is shown. (F,G) Assessment of mitophagy in vehicle- and tomatidine-treated primary rat cortical neurons. Colocalization between the mitophagy ...
参考价¥1 B16-F10-GFP细胞 B16-F10-GFP黑色素瘤细胞 参考价¥1 产品分类品牌分类 复苏细胞 冻存细胞胃腺癌细胞卵巢癌细胞N87细胞肝癌细胞TE-1细胞鼻咽癌细胞HeLa细胞淋巴瘤细胞 全部产品列表 产品简介 SKNSH人神经母细胞瘤细胞 SKNSH细胞形态,细胞系:严格无菌操作,鉴定,可传代次数好,附培养操作说明和注意事项...
In the ChAT-eGFP mouse, both GFP-positive (GFP+; ChAT/Trpm5-expressing) microvillous cells and DAPI-stained nuclei in the most superficial layer of the MOE above the nuclei of supporting cells were counted manually. To avoid including supporting cells in the count, we counted only nuclei ...