fast-skeletal, and cardiac isoforms (ssC1C2, fsC1C2, and C0C2, respectively) were generated, purified, and analyzed with SDS-PAGE41(Fig.1). Each MyBP-C isoform was expressed using a pET28 expression system (Millipore 70777) inE.coliand included an N-terminal His-tag. Protein expression...
Over the last 3 h, unlabeled amino acids are also infused. This provides addi- tional substrate for muscle protein synthesis; normally, what is seen is a spike in synthetic rate. However, inevitably, mus- cle protein breakdown exceeds muscle protein synthesis.10 Both endogenous glucocorticoids ...
Over the last 3 h, unlabeled amino acids are also infused. This provides additional substrate for muscle protein synthesis; normally, what is seen is a spike in synthetic rate. However, inevitably, muscle protein breakdown exceeds muscle protein synthesis.10 Both endogenous glucocorticoids and pro-...
Competition experiments using a 50-fold excess of unlabeled ARERNA are also shown (ARE-C). HuR Binds the AChE 3′-UTR—Our findings usingREMSA illustrate the importance of ARE in the binding of cytoplasmic factorsto the AChE 3′-UTR. Members of the ELAV-like family of mRNA-bindingproteins...
at the 5'-enhancer as well as at theMCK-SIE (Figure4B). These data demonstrate that a rapid and complete MyoD-to-myogenin binding transition is not observed in the cell culture system used in our study. However, it may be informative that we found the ratio of myogenin to MyoD ...
As a control for binding specificity, a nonspecific competitor probe must be used to show that the unlabeled nonspecific probe cannot sequester/compete for the protein away from the labeled probe. We used the unlabeled MEF2-probe as the competitor and the unlabeled mut MEF2-probe as a ...