Complete and detailed, this Skeletal System Coloring Workbook is perfect for instruction, study and even testing high school level students.
Next, we collected flow-through contained unlabeled cells that are enriched for satellite cells. Cell lines generated from these cells are referred as RH-hMuSC-1, RH-hMuSC-2, RH-hMuSC-3, and RH-hMuSC-4 representing samples 1, 2, 3 and 4, respectively. HMuSCs were centrifuged, ...
fast-skeletal, and cardiac isoforms (ssC1C2, fsC1C2, and C0C2, respectively) were generated, purified, and analyzed with SDS-PAGE41(Fig.1). Each MyBP-C isoform was expressed using a pET28 expression system (Millipore 70777) inE.coliand included an N-terminal His-tag. Protein expression...
(A) Diagram of the 6.5-kb MCK regulatory region with the three known active regulatory regions: the 5'-enhancer, PP, MR1 (white boxes), the MCK-SIE (light gray box) exons 1 and 2 (black boxes) and other regions (gray), including the 33.7-kb Mark4 gene (located approximately 40 ...
Competition experiments using a 50-fold excess of unlabeled ARERNA are also shown (ARE-C). HuR Binds the AChE 3′-UTR—Our findings usingREMSA illustrate the importance of ARE in the binding of cytoplasmic factorsto the AChE 3′-UTR. Members of the ELAV-like family of mRNA-bindingproteins...
Over the last 3 h, unlabeled amino acids are also infused. This provides addi- tional substrate for muscle protein synthesis; normally, what is seen is a spike in synthetic rate. However, inevitably, mus- cle protein breakdown exceeds muscle protein synthesis.10 Both endogenous glucocorticoids ...
Over the last 3 h, unlabeled amino acids are also infused. This provides additional substrate for muscle protein synthesis; normally, what is seen is a spike in synthetic rate. However, inevitably, muscle protein breakdown exceeds muscle protein synthesis.10 Both endogenous glucocorticoids and pro-...
The formation of complex 4a and complex 4b in an EMSA with an oligonucleotide probe of the mAT1 region could be totally inhibited by competition with an excess of unlabeled oligonucleotide (MHox control, modified) containing an MHox-binding site (from the mck enhancer (35), but with a point...
As a control for binding specificity, a nonspecific competitor probe must be used to show that the unlabeled nonspecific probe cannot sequester/compete for the protein away from the labeled probe. We used the unlabeled MEF2-probe as the competitor and the unlabeled mut MEF2-probe as a ...
International Journal of Molecular Sciences Article PGC-1α and MEF2 Regulate the Transcription of the Carnitine Transporter OCTN2 Gene in C2C12 Cells and in Mouse Skeletal Muscle Katerina Novakova 1, Michael Török 1, Miljenko Panajatovic 1, Jamal Bouitbir 1,2 , François H. T. Duong...