Digoxigenin-labelled anti-sense RNA probes were produced using PCR product as template, and the SP6/T7 RNA polymerase Riboprobe Combination System (Promega), in accordance with the manufacturer’s instructions. The probes were subsequently purified using mini Quick Spin RNA Columns (Roche). Primer ...
A major purpose of nuclear and mitochondrial signalling crosstalk is to achieve a well-developed and functional mitochondrial organelle system within the cell. Such signalling involving the nucleus is governed via protein translocation and involves the movement of numerous proteins to/from the nucleus to...
However, this was questioned based on results obtained from INS-1β and HEK cells overexpressing GLP1R in which obestatin was not able to displace radiolabelled GLP-1 binding42 and the fact that this work is prior to the report about quality of obestatin peptides. Furthermore, to the best ...
For flow cytometry, cells were labelled with propidine iodide (PI) and Annexin V according to the manufacturer's instructions (KENGEN Biotech). The methods for calculating the rate of apoptosis were provided by FlowJo software. 2.7. Detection of antioxidant function To identify the effect of ...
From ~6 to 12 months of age, TSCmKO display a progressive sarcopenia-like phenotype including muscle atrophy and weakness as well as accumulation of p62-labelled proteins and aggregates33, which are also, to some degree, features of sarcopenia13,34. We therefore tested whether 7 months of ...
tdTomato. Insets 1,2 and 3 show magnified views of the corresponding areas (labelled with 1, 2, 3). Bottom left, Sox9 staining of primary Pthrp-creER tdTomato+ colonies. Red, tdTomato. Scale bars, 5 mm (top panels), 1 mm (top panel insets), 200 μm (bottom panel). n...
They were then preincubated with 1% BSA in 50 mM Tris–HCL, pH 8.2 for 10 min at RT and labelled with a goat anti mouse IgG gold-conjugated 10 nm, (Tebu bio, France) diluted 1/80 in 1% BSA-, 50 mM Tris–HCL, pH 8.2 in a wet chamber for 45 min. Grids were successively ...
DNA was labelled with Hoechst (blue). A representative picture of an MuSC is shown. Scale bar = 5 μm. (b) Setdb1 knockdown in MuSCs. Freshly isolated EDL mouse single myofibres were transfected with control (siCTRL) or Setdb1 siRNA (siSetdb1) 2 h after isolation and cultured for ...
Regular exercise elicits advantageous metabolic adaptations in skeletal muscle, such as improved insulin sensitivity. However, the underpinning molecular mechanisms and the effect of diet on muscle exercise training benefits are unclear. We therefore cha
(IF). DNA was labelled with Hoechst (blue). A representative picture of an MuSC is shown. Scale bar=5 μm. (b) Setdb1 knockdown in MuSCs. Freshly isolated EDL mouse single myofibres were transfected with control (siCTRL) or Setdb1 siRNA (siSetdb1) 2 h after isolation and ...