Plectin interaction with the microfilament system was demonstrated by ultrastructural studies using immunogold-labeled antibodies to plectin. When whole mount electron microscopy was applied to glioma C6 cells, plectin was detected as thin (3-nm) filamentous structures that linked IFs (vimentin) to ...
Fig. 6 Functional communities of chromatin-chromatin and RNA-chromatin interaction network.aThe network of all functional communities, some communities are highlighted and labeled.bOne community harboring 9 genes out of 54 GWAS-detected genes (3 growth-associated genes and 6 meat quality-related genes)...
The peak pulsed-wave TDI velocity amplitude of the ejection phase and early and late diastole were measured off-line using a computer-assisted drawing system, and the values were expressed in cm/s (10). Five consecutive beats were analyzed, and the mean velocity values were calculated to ...
Clades in the resulting phylogenetic tree were labeled using the system employed in a recent analysis (U-taynapun et al. 2011). rically in linear rows (Fig. 1A) located at the position of the proximal pterygiophores (1 to 2 cm from the fin base), which support the fin rays (both ...
we used a standardized positioning system for mice and probe, controlling muscle length and without any pressure exerted by the probe. It allowed measuring muscle viscoelastic properties at different muscle lengths and confirmed that passive stretch-induced stiffening correlates with fibrosis in skeletal ...
Northern blot analysis of mouse tissues was done using digoxigenin-labeled RNA probes, as described (72). A fragment of mouse Carns1 cDNA (nucleotides 424–1254) was amplified by PCR, using primers shown in Table 1, and subcloned into pBluescript SK(−). Digoxigenin-labeled RNA antisense...
Aorta-derived single cells were labeled with PE-conjugated rat anti-mouse CD31 (BD Bioscience) and rabbit anti NG2 / secondary anti rabbit IgG FITC (Sigma) and then sorted in MoFlo System (DAKO). Alkaline Phosphatase Staining Fixed cells were incubated in alkaline buffer containing NBT/BCIP ch...
The CSA of muscle fiber was determined by manually drawing contours around individual fibers using ZEN 2 (blue edition) software (Carl Zeiss). For immunofluorescence examination, sections were incubated with FITC-labeled wheat germ agglutinin to stain sarcolemma. 4.8. Statistical Analysis Results are ...