Digoxigenin-labelled anti-sense RNA probes were produced using PCR product as template, and the SP6/T7 RNA polymerase Riboprobe Combination System (Promega), in accordance with the manufacturer’s instructions. The probes were subsequently purified using mini Quick Spin RNA Columns (Roche). Primer ...
A major purpose of nuclear and mitochondrial signalling crosstalk is to achieve a well-developed and functional mitochondrial organelle system within the cell. Such signalling involving the nucleus is governed via protein translocation and involves the movement of numerous proteins to/from the nucleus to...
In a human context in vitro, the obestatin/GPR39 system exerts an autocrine effect on the skeletal myogenic process promoting myogenic differentiation and fusion of human myoblasts11. At the molecular level, the action of obestatin involves both G-protein-dependent and -independent mechanisms linking...
from Blaiset al.[40] For significance Student’s pairedt-test was applied. *P-values <0.05. (d) Venn diagram showing the overlap between Setdb1/
For flow cytometry, cells were labelled with propidine iodide (PI) and Annexin V according to the manufacturer's instructions (KENGEN Biotech). The methods for calculating the rate of apoptosis were provided by FlowJo software. 2.7. Detection of antioxidant function To identify the effect of ...
During differentiation, the plasma membrane of the myotube folds into the cell-forming caveolae, which leads to the formation of an extended transcellular web of surface membranes, named the transverse tubule (TT) system [20], which creeps into the muscle fibre, increasing the total surface area...
Protein was solubilised in SDS buffer and protein thiols were labelled with the fluorescent dye BODIPY FL-N-(2-aminoethyl) maleimide (FLM, Invitrogen, Waltham, MA, USA). Following the removal of the unbound dye using cysteine, protein was re-solubilised in SDS buffer and oxidised thiols ...
Biological hierarchy of the neuromuscular system. Shown are the organisation of the genome, transcriptome and proteome of motor neurons and skeletal muscles. The histological image illustrates neuromuscular junctions on individual muscle fibres labelled for the presence of the enzyme acetylcholinesterase. Full...
chaperones are known to interact49, our data indicate a coordinated increase in the protein quality control system in response to exercise training. While we detected many subunits of the translocases of the outer and inner membrane (TOM and TIM, respectively; Supplementary Data3), which are requ...
tdTomato. Insets 1,2 and 3 show magnified views of the corresponding areas (labelled with 1, 2, 3). Bottom left, Sox9 staining of primary Pthrp-creER tdTomato+ colonies. Red, tdTomato. Scale bars, 5 mm (top panels), 1 mm (top panel insets), 200 μm (bottom panel). n...