Cranial sutures are major growth centers for the calvarial vault, and their premature fusion leads to a pathologic condition called craniosynostosis. This study investigates whether skeletal stem/progenitor cells are resident in the cranial sutures. Prospective isolation by FACS identifies this population ...
We first confirm that skeletal stem/progenitor cells (referred to as CD51+;CD200+cells) are resident in cranial sutures and validated their multipotency and ability to self-renew. CD51+;CD200+cells are then prospectively isolated from physiologically fusing and patent sutures, in addition to cra...
The stem or progenitor cells responsible for tissue homeostasis have been identified in many organs. Some of them, such as hematopoietic or intestinal epithelium stem cells, are multipotent and can differentiate into various cell types. Others are unipotent. The skeletal muscle tissue does not self-...
Skeletal stem and progenitor cells (SSPCs) perform bone maintenance and repair. With age, they produce fewer osteoblasts and more adipocytes leading to a loss of skeletal integrity. The molecular mechanisms that underlie this detrimental transformation a
Conversely, the aged SSC lineage promoted osteoclastic activity and myeloid skewing by haematopoietic stem and progenitor cells, suggesting that the ageing of SSCs is a driver of haematopoietic ageing. Deficient bone regeneration in aged mice could only be returned to youthful levels by applying a ...
Single-Cell Transcriptome Analysis of Growth Plate and Diaphysis Reveals Markers for hSSC Isolation As described above, our findings in mice (Chan et al., 2015b, Marecic et al., 2015) suggested that similar types of lineally related skeletal stem and progenitor cells may also be prospectively ...
Stem cells, cells that maintain their ability to replicate and can differentiate into various cell types, have been important in understanding cell regulation. In addition, these cells are used therapeutically with continued research hoping to increase their therapeutic potential. Like many other organs...
aging; bone marrow adipocytes; bone marrow stromal cells (BMSCs); bone regeneration; lineage-tracing; osteoporosis; single-cell RNA-sequencing (scRNA-seq); skeletal stem and progenitor cells (SSPCs)1. Introduction Progressive aging has a substantial negative impact on the skeletal system. Diseases ...
The tissue-resident skeletal stem cells (SSCs), which are self-renewal and multipotent, continuously provide cells (including chondrocytes, bone cells, marrow adipocytes, and stromal cells) for the development and homeostasis of the skeletal system. In recent decade, utilizing fluorescence-activated cel...
The tissue-resident skeletal stem cells (SSCs), which are self-renewal and multipotent, continuously provide cells (including chondrocytes, bone cells, marrow adipocytes, and stromal cells) for the development and homeostasis of the skeletal system. In recent decade, utilizing fluorescence-activated cel...