HOMA2-IR was calculated using the HOMA2 Calculator (v.2.23, University of Oxford) available online at https://www.dtu.ox.ac.uk/homacalculator/. After an overnight fast, percutaneous vastus lateralis muscle biopsies (Weil-Blakesley conchotome) containing between 10 and 60 mg of the tissue ...
Transdifferentiation of human non-muscle cells directly into myogenic cells by forced expression of MyoD represents one route to obtain highly desirable human myogenic cells. However, functional properties of the tissue constructs derived from these transdifferentiated cells have been rarely studied. Here,...
Preserving skeletal muscle function is essential to maintain life quality at high age. Calorie restriction (CR) potently extends health and lifespan, but is largely unachievable in humans, making “CR mimetics” of great interest. CR targets nutrient-sensing pathways centering on mTORC1. The mTORC1...
The temporal expression pattern of circular RNAs (circRNAs) across developmental stages is essential for skeletal muscle growth and functional analysis. However, there are few analyses on the potential functions of circRNAs in rabbit skeletal muscle development. Results Initially, the paraffin sections sho...
AAS: adjusted activity score; BMI: body mass index; HAP: human activity profile; SD: standard deviation. 3.2. Reliability of Measures of Muscle Hydration Results for the measures of hydration for time one and two are presented as group means and standard deviations in Table2. Values for the ...
Long intergenic non-coding RNAs (lincRNAs) play essential roles in numerous biological processes and are widely studied. The skeletal muscle is an important tissue that plays an essential role in individual movement ability. However, lincRNAs in pig skel
liberating calcium and phosphate in response to endocrine demands4. These features allow the osteocyte network to maintain both the structural integrity of the skeleton and mineral homeostasis. Osteocytes also have regulatory functions beyond the skeleton, including in skeletal muscle, adipose tissue, the...
LncRNAs are essential for regulating skeletal muscle. However, the expression profile and function of lncRNAs in goat muscle remains unclear. Here, an average of ~14.58 Gb high-quality reads were obtained from longissimus dorsi tissues of 1-month-old (n
Identification and Characterization of Long Non-Coding RNA in Sheep Muscle To study the basic features of lncRNAs in sheep muscle, the lncRNAs were identified and compared with mRNAs. The intersection of the Coding Potential Calculator, Coding-Non-Coding IndeGxen,etsh2e017P,r8o, 1t9e1in ...
The mCT data files were quantified using the Xelis 3D-Image calculator software 2.0 (Infinitt, Seoul, Republic of Korea). 2.9. Tissue Staining Gastrocnemius muscle tissues were isolated and fixed with 10% neutral buffered formalin for 1 day. Paraffin sections 5 μm-thick were sliced and stained...