Fig 3Analysis of HER2 mRNA expression by RT PCR 2.3细胞HER2蛋白表达水平与未转染组相比,转染pSilencer HER2重组质粒的细胞中HER2蛋白的表达明显受到抑制,而转染无义质粒组细胞HER2的表达没有明显降低,结果与RT PCR结果一致(图4)。 1:SKBr3未转染组; 2:无义质粒组; 3:pSilencer HER2转染后24 h; 4:pSilen...
Fig 3Analysis of HER2 mRNA expression by RT PCR 细胞HER2蛋白表达水平与未转染组相比,转染pSilencer HER2重组质粒的细胞中HER2蛋白的表达明显受到抑制,而转染无义质粒组细胞HER2的表达没有明显降低,结果与RT PCR结果一致(图4)。 1:SKBr3未转染组; 2:无义质粒组; 3:pSilencer HER2转染后24 h; 4:pSilencer...
caneffectivelyinhibittheexpressionofHER2geneandthe proliferationofthecellsinvitro. 【Keywords】HER2;RNAinterference;genetherapy 0引言 目前,乳腺癌癿死亡人数占到女性肿瘤癿40%[1].研究表明许多肿 瘤癿収生都不特定癌基因戒酪氨酸激酶叐体基因过表达导致癿细胞增殖有关. HER2叐体已被证实是不乳腺肿瘤収生密切相关...
The results from infection of SKBR3 cells with siRNA-expressing lentivirus demonstrated that lentiviral-mediated RNAi could downregulate HER2 expression efficiently through fluorescent quantitative PCR (FQ-PCR), western blot, and FACS analysis. Furthermore, cell growth was inhibited in cell proliferation ...
HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212 Hudziak RM, et al. Monoclonal antibodies directed to the Her2 receptor. US Patent 5,677,171 dated Oct 14 1997 细胞图片: SK-BR-3...
.There was no appar-ent change in HER2protein expression after herceptin treatment (F =0.973,P >0.05).Co-immunoprecipitation showed coprecipita-tion between the Notch-1and HER2proteins.Conclusion:The activity of Notch-1protein was decreased in the breast cancer cells with HER2overexpression.HER2...
In addition, we found that the magnitude of the upregulation is highly subtype-specific, being the HER2+ cells the model with lowest HOXB7 upregulation, presenting very low or even null expression for HOXB8 and HOXB9, respectively. These results are analyzed in more detail in “HOX genes ...
Mahmoodi, N.; Motamed, N.; Paylakhi, S.H.; Mahmoodi, N.O. Comparing the effect of silybin and silybin advanced (tm) on viability and HER2 expression on the human breast cancer SKBR3 cell line by no serum starvation. Iranian J. Pharm. Res. 2015, 14, 521-530....
The EGFR and HER2 expression of A431, U343, SKOV3 and SKBR3 cells in four culturing conditions.Hanna BjörkelundLars GeddaPavel BartaMagnus MalmqvistKarl Andersson
Theadenovirus-mediatedRNAicoulddownregulatetheHER2expressioneficientlyandex-ertaninhibitoryefectongrowthofHER2-overexpressing收稿日期:2006—10—12;接受日期:2006—12—29基金项目:国家自然科学基金资助项目(30400078);国家重点基础研究发展规划(973)项目(2002CB713700);安徽省自然科学基金资助课题(O5o43O201)作者简介:...