Fig 3Analysis of HER2 mRNA expression by RT PCR 2.3细胞HER2蛋白表达水平与未转染组相比,转染pSilencer HER2重组质粒的细胞中HER2蛋白的表达明显受到抑制,而转染无义质粒组细胞HER2的表达没有明显降低,结果与RT PCR结果一致(图4)。 1:SKBr3未转染组; 2:无义质粒组; 3:pSilencer HER2转染后24 h; 4:pSilen...
Fig 3Analysis of HER2 mRNA expression by RT PCR 细胞HER2蛋白表达水平与未转染组相比,转染pSilencer HER2重组质粒的细胞中HER2蛋白的表达明显受到抑制,而转染无义质粒组细胞HER2的表达没有明显降低,结果与RT PCR结果一致(图4)。 1:SKBr3未转染组; 2:无义质粒组; 3:pSilencer HER2转染后24 h; 4:pSilencer...
caneffectivelyinhibittheexpressionofHER2geneandthe proliferationofthecellsinvitro. 【Keywords】HER2;RNAinterference;genetherapy 0引言 目前,乳腺癌癿死亡人数占到女性肿瘤癿40%[1].研究表明许多肿 瘤癿収生都不特定癌基因戒酪氨酸激酶叐体基因过表达导致癿细胞增殖有关. HER2叐体已被证实是不乳腺肿瘤収生密切相关...
The results from infection of SKBR3 cells with siRNA-expressing lentivirus demonstrated that lentiviral-mediated RNAi could downregulate HER2 expression efficiently through fluorescent quantitative PCR (FQ-PCR), western blot, and FACS analysis. Furthermore, cell growth was inhibited in cell proliferation ...
HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212 Hudziak RM, et al. Monoclonal antibodies directed to the Her2 receptor. US Patent 5,677,171 dated Oct 14 1997 细胞图片: SK-BR-3...
.There was no appar-ent change in HER2protein expression after herceptin treatment (F =0.973,P >0.05).Co-immunoprecipitation showed coprecipita-tion between the Notch-1and HER2proteins.Conclusion:The activity of Notch-1protein was decreased in the breast cancer cells with HER2overexpression.HER2...
方法Western印迹法及抗体介导的特异性细胞增殖试验;筛选乳腺癌病人抗HER2/neu抗体阳性血清IgG的ELISA方法。结果试验发现单抗c-neu-5对SKBR3细胞p185酪氨酸磷酸化有明显促进作用,但对细胞增殖的影响不明显;而单抗c-neu-2对SKBR3细胞p185酪氨酸磷酸化及细胞增殖均有明显作用。在此基础上,收集乳腺癌病人血清14份,分离...
In addition, we found that the magnitude of the upregulation is highly subtype-specific, being the HER2+ cells the model with lowest HOXB7 upregulation, presenting very low or even null expression for HOXB8 and HOXB9, respectively. These results are analyzed in more detail in “HOX genes ...
Mahmoodi, N.; Motamed, N.; Paylakhi, S.H.; Mahmoodi, N.O. Comparing the effect of silybin and silybin advanced (tm) on viability and HER2 expression on the human breast cancer SKBR3 cell line by no serum starvation. Iranian J. Pharm. Res. 2015, 14, 521-530....
We have previously identified differentially expressed proteins with antigenic activity between SKBR3 (ER-, high HER2 expression) and MCF7 (ER+, low HER2 expression) breast cancer cell lines. The aims of the present study were (1) to develop an initial proteome based roadmap of differentially ...