The integration of graphene materials into electrochemical biosensing platforms has gained significant interest in recent years. Bulk quantities of graphene can be synthesized by oxidation of graphite to graphite oxide and subsequent exfoliation to graph
Successful assembly and insertion of the nanopore into lipid bilayers are validated by transmission electron microscopy (TEM), while selective translocation of cargo and the pore mechanosensitivity are studied using optical methods, including single-molecule, total internal reflection fluorescence (TIRF) ...
Comparison of uptake of different PNP 45 [[4]] and PNP 90 in NR8383 and Caco-2 cells (1 μg/ml) as % of unperturbed uptake upon 24 h exposure after blocking the endocytotic uptake (n = 5) at 4 ºC or by a mixture of 2-deoxyglucose and sodium azide. The * sign signi...
Polymer nanoparticles (PNP) are becoming increasingly important in nanomedicine and food-based applications. Size and surface characteristics are often considered to be important factors in the cellular interactions of these PNP, although systematic inve
A component of CF, countin, affects group size in a manner similar to CF, and a 1-min exposure of cells to countin decreases glucose levels. Adding 1 mm exogenous glucose negates the effect of high levels of extracellular CF on group size and mimics the effect of depleting CF on ...
Small anti-PECAM/DNA conjugates transfected target cells in culture 5-fold more effectively than their large counterpart (350- versus 4200-nm diameter). To evaluate the practical significance of the size-controlled subcellular addressing, we coupled glucose oxidase (GOX) to anti-PECAM and anti...
Rice grain size (GS) is an essential agronomic trait. Though several genes and miRNA modules influencing GS are known and seed development transcriptomes analyzed, a comprehensive compendium connecting all possible players is lacking. This study utilizes
L6 myoblasts were maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 4.5 g/l glucose, supplemented with 10% (v/v) fetal bovine serum at 37°C and 5% CO2. To induce differentiation, cells were seeded at a density of 5.105 cells per well in 6-well plates, grown to confluenc...
Grain size is an important component trait of grain yield, which is frequently threatened by abiotic stress. However, little is known about how grain yield and abiotic stress tolerance are regulated. Here, we characterize GSA1, a quantitative trait locus
In the reaction, 1 3 106 cells were reacted with 3 3 105 magcups in binding buffer27, consisting of Dulbecco's PBS containing 25 mM glucose, 5 mM MgCl2, and 1% BSA, for 30 min at room temperature with gentle mixing every 10 min. After the reaction, the sample was washed with ...