It has been known since the earliest studies on the biosynthesis of proteins that the ribosomal particles are the sites of protein synthesis in the cell. These nucleoprotein particles, present in every cell which carries out protein synthesis, vary somewhat in size but are of basically similar ...
To increase the specificity of these labeling reactions, three broad strategies have emerged: (1) those that utilize reactive small-molecule chemical reagents to modify endogenous proteins; (2) those that harness or hijack protein translational processes for direct labeling; and (3) those that ...
Recognition of the T stem-loo... A Loria,P Tao - 《Biochemistry》 被引量: 139发表: 1998年 Inhibition of hepatitis C virus replication and internal ribosome entry site-dependent translation by an RNA molecule. Hepatitis C virus (HCV) protein synthesis is mediated by a highly conserved ...
A new strategy for the synthesis of glycoproteins. Science 303, 371–373 (2004). Article CAS Google Scholar Xu, R. et al. Site-specific incorporation of the mucin-type N-acetylgalactosamine-β-O-threonine into protein in Escherichia coli. J. Am. Chem. Soc. 126, 15654–15655 (2004). ...
anneals to theviral RNA(70S or heat-denatured35SRNA) over a 5′-end region of ∼18 nt, calledpbs(primer-binding site).AMV RTasehas abinding sitefor tRNATrp, and the tRNA binds with the highest affinity of all chicken cell tRNAs. AMV RTase can bind the tRNA in theabsenceoftemplate...
Bifunctionality of dirhodium tetracarboxylates in metallaphotocatalysis ArticleOpen access10 November 2023 A cysteine selenosulfide redox switch for protein chemical synthesis ArticleOpen access22 May 2020 Introduction Proteins bearing post-translational modifications (PTMs) or designer modifications, such as ...
G-protein-biased agonism of the mu-opioid receptor (μ-OR) is emerging as a promising strategy in analgesia. A deep understanding of how biased agonists modulate and differentiate G-protein-coupled receptors (GPCR) signaling pathways and how this is transferred into the cell are open questions....
Towards a Biocatalyst for (S)-Styrene Oxide Production: Characterization of the Styrene Degradation Pathway of Pseudomonas sp. Strain VLB120 In order to design a biocatalyst for the production of optically pure styrene oxide, an important building block in organic synthesis, the metabolic pathwa......
carbohydrates bind to and act on the protein therapeutic targets has hampered the translation of carbohydrates to the clinic9. It is difficult to obtain experimental data about carbohydrate-protein interactions, due to the structural diversity of carbohydrates. For example, the most widely used ...
For example, we have shown that MG is a partial MOR agonist (mu-opioid receptor agonist) with a potential bias for G protein signaling (showing no β-arrestin-2 recruitment) in cell-based assays15,16. Further, we have found that 7OH is a more potent and efficacious partial MOR agonist ...