Proteins whose functionality is not well characterized form a large percentage of entries in many of the currently available biological databases, including the Protein Data Bank (PDB), and there is a constantly growing demand for reliable and fast synthesis and characterization methods. When it comes...
Cell-free protein synthesis (CFPS) systems comprise a large repertoire of biochemical pathways that can easily be controlled and manipulated9. Recent examples are (i) the directed incorporation of non-canonical amino acids into proteins at multiple sites6, (ii) the construction and characterization o...
as they require a host cell for their replication. We investigated the use of an axenic medium forE.chaffeensisgrowth, assessed by protein and DNA synthesis, in the absence of
Size,distribution,andvulnerabilityoftheglobalsoilinorganiccarbon 中国科学院大学,等 (张甘霖,等) Science 4/12 13 TheHEATrepeatproteinHPO-27isalysosomefissionfactor 南方科技大学、中国科学院大 学,等 (王晓晨、冯巍) Nature 4/18 14 Anaturallyisolatedsymbiotic bacteriumsuppressesflavivirus transmissionbyAedesmosquit...
PTMs occur during or after protein synthesis. They change the physicochemical properties and potentially the activity of the protein. They range from small chemical modifications to the amino acid chain, to the addition of complex branching structures on the protein backbone [32,33]. Such modificati...
cell-free protein synthesis;chassis;gene circuits;micro-compartments;stochasticity 1. Introduction Fascinated by the emergence of life from non-living matter through billions of years of evolution, scientists began to comprehend and reconstruct how this occurred. Advances in multi-disciplinary research fiel...
However, they take part in a number of other processes, whether physiological or pathological. They mediate in mineral homeostasis, synthesis of steroids, or even apoptosis [176,177]. Although these are crucial for the cell’s maintenance or even the homeostasis of a whole organism, their most...
The journal is divided into the following editorial sections: Metabolic engineering Synthetic biology Whole-cell biocatalysis Microbial regulations Recombinant protein production/bioprocessing Production of natural compounds Systems biology of cell factories Microbial production processes Cell-free systems Calls for...
Studies of a surface protein called GP115 (Gas1p) led to the discovery that it was a major yeast GPI protein, and subsequent work has used this protein as a subject for GPI synthesis, processing, and transport [47] (Chapter 13). The association of GPI proteins with cell walls came ...
(Fig.6). RNA editing, as a co/posttranscriptional modification, can change nucleotide sequences and have important effects on viral replication, protein synthesis, infection, and virulence. ADARs and APOBECs, in addition to deamination, are RNA-binding proteins that directly interact with endogenous...