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Cell-free protein synthesisallows to rapidly generate functional proteins independent of cell culture. Although advances in eukaryotic lysates have increased the amount of protein that can be produced, the nuances of different translation systems lead to variability in protein production (Carlson et al....
Cell-free protein synthesis (CFPS) systems derived from crude cell extracts have been used for decades as a research tool in fundamental and applied biology (Fig. 1). They were used in the ground-breaking experiments of Nirenberg and Matthaei (1961), playing an essential role in the discovery...
Cell-free protein synthesis has been developed as a critical platform in synthetic biology. Unlike the cell-based synthesis system, cell-free system activates transcriptional and translational mechanisms<italic>in vitro </
Cell-free protein synthesis is used to produce proteins with various structural traits. Recent bioinformatics analyses indicate that more than half of eukaryotic proteins possess long intrinsically disordered regions. However, no systematic study concerning the connection between intrinsic disorder and expressi...
Cell-free protein synthesis is a versatile protein production system. Performance of the protein synthesis depends on highly active cytoplasmic extracts. Extracts from E. coli are believed to work best; they are routinely obtained from exponential growin
Answer to: Describe the process of protein synthesis in a cell. Correctly include the following terms: ribosome, t-RNA, DNA, m-RNA, amino acid,...
The journal is divided into the following editorial sections: Metabolic engineering Synthetic biology Whole-cell biocatalysis Microbial regulations Recombinant protein production/bioprocessing Production of natural compounds Systems biology of cell factories ...
Specific pathways and protein interactions of TK1 with other factors that lead to tumor cell progression have not been extensively explored. Fully elucidating the mechanisms behind TK1 elevation in cancer cells and its correlation to cancer progression begins with understanding the regulatory mechanisms of...
23 lauryl ether in an amount of 0.02 to 0.2% by volume and a lipid; and(b) decreasing the concentration of said detergent in the reaction mixture simultaneously with the protein synthesis or after a period therefrom,wherein the synthesized protein has at least a part of its biological ...