(1988) The metaphase scaffold is helically folded: sister chromatids have predominantly opposite helical handedness. Cell , 55 , 937–44.Boy De La Tour E, Laemmli UK (1988) The metaphase scaffold is helically folded: sister chromatids have predominantly opposite helical handedness. Cell 55:937...
In contrast, when DNA has been replicated during S-phase, the sister chromatids can be used as templates for error-free repair through homologous recombination (HR)4. Cyclin-dependent kinase (CDK)-mediated activation of CtIP promotes the resection of the broken DNA ends by BRCA1/BARD1 and ...
Our current results show that the oocytes from XY females went through the first meiotic division and assembled and positioned the MII-spindles normally during maturation in vivo or in vitro. Furthermore, the sister-chromatids were separated after activation while the second polar bodies were extrude...
Two essential steps in chromosome segregation are the establishment of bivalent microtubule attachments in metaphase and the dissolution of sister cohesion at anaphase. Sister chromatids become linked together during DNA replication and bind in pairs to microtubules via kinetochores, DNA-protein complexes ...
Human metaphase chromosomes, in which 5-bromo-deoxyuridine (BrdU) had been incorporated into the DNA, were treated with the fluorescent plus Giemsa (FPG) method. Use of this method distinctly stained one of the paired sister chromatids with the Giemsa solution due to the difference in content ...
SeparationofSisterChromatidsinMitosis RequirestheDrosophilapimplesProduct,aProtein DegradedaftertheMetaphase/AnaphaseTransition RembertStratmannandChristianF.Lehnerwithinthecentromericregion,isessentialfortheor- dereddistributionofhomologsandsistersinmeiosisIFriedrich-Miescher-Laboratorium derMax-Planck-GesellschaftandII(Gol...
Cohesion between sister chromatids in vertebrates is lost in two steps. First, during prophase and prometaphase, the bulk of vertebrate Scc1 dissociates from chromosome arms following phosphoryation by polo kinase(Sumara et al., 2002). Chromatids separate completely at anaphase when vertebrate sepa...
Dual inhibition of sister chromatid separation at metaphase. Cell 107 (6), 715-726.Stemmann, O., Zou, H., Gerber, S. A., Gygi, S. P. & Kirschner, M. W. 2001 Dual inhibition of sister chromatid separation at metaphase. Cell 107, 715-726....
Lehner, 1996 Separation of sister chroma- tids in mitosis requires the Drosophila pimples product, a protein degraded after the metaphase/anaphase transition. Cell 84: 25-35.Stratmann, R., and C.F. Lehner. 1996. Separation of sister chromatids in mitosis requires the Drosophila pimples product,...
AID-tagging often reduces protein levels already before the addition of auxin59,60, which for NIPBL might impair sister-chromatid cohesion establishment during S-phase. We therefore performed metaphase congression analysis by time-lapse microscopy of WT HeLa cells, HeLa Sororin-AID cells and HeLa ...