网络阴性对照 网络释义 1. 阴性对照 本试验用Ambion公司设计软件设计并合成3条含21个碱基的siRNA(siRNAl,siRNA2,siRNA3)及随机阴性对照(siRNAcon)。在S… d.wanfangdata.com.cn|基于 1 个网页
The invention discloses a maize dwarf mosaic virus and maize rough dwarf virus-resistant selectable marker-free siRNA composite expression vector and a construction method thereof. The method comprises the following steps of: respectively amplifying specific fragments of coat protein genes of maize dwarf...
U2OS, mTPX2, mTPX2, and mTPX2 cells transfected with TPX2 or CON siRNA were treated on ice to completely depolymerize microtubules, transferred to 37掳C for 90 s to allow ...
The siRNA can be prepared in many ways such as chemical synthesis, in vitro transcription, enzyme cleavage of long-chain dsRNA, vector expression of siRNA, PCR synthesis of siRNA expression elements. The presence of these methods provides a selection space for researchers and can be used to obta...
The promotion of siRNA delivery to breast cancer overexpressing epidermal growth factor receptor through anti-EGFR antibody conjugation by immunoliposomes. Biomaterials, 32(13), pp.3459-70.Gao J, Liu W, Xia Y, Li W, Sun J, Chen H, et al. The promotion of siRNA delivery to breast cancer...
All the carbohydrate-siRNA derivatives were compatible with RNA interference machinery if transfected with oligofectamine. In the absence of a transfection agent, some of them exerted certain reduction of gene expression. Double-tailed permethylated glucose conjugated to siRNA...
Jie Gao,Wei Liu,Yu Xia,Wei Li,Jing Sun,Huaiwen Chen,Bohua Li,Dapeng Zhang,Weizhu Qian,Yanchun Meng,Li Deng,Hao Wang,Jianming Chen,Yajun Guo.The promotion of siRNA delivery to breast cancer overexpressing epidermal growth factor receptor through anti-EGFR antibody conjugation by immunoliposomes[...
After 48 h, cells were analyzed by branched-DNA RNA assay for CTNNB1 mRNA. CTNNB1 levels for a given sample were normalized to PPIB levels for that sample.Philip L. Lorenzi
Test inhibition of GAPDH expression through siRNA in rat mesenchymal stem cellsAmalia ForteNicola AlessioMauro FunicelliUmberto GalderisiVia L. De Crecchio