首先DSIR在“design name”的对话框中必须输入基因编号(来自NCBI),然后就是“sequence”它要求“FASATA format only”,其实这种格式就是要求在基因序列前边加入“>”(英文状态大于符号)即可,最后点击Run analysis即可。 (图4 DSIR siRNA基本参数简介) (4)DSIR引物的选择 首先,因siRNA作用的具体位置目前还不确定,因此...
This tool generates a negative control for siRNA as a scrambled sequence of the siRNA target sequence.
注意NCBI-BLAST 上的sequence ID要和NCBI-Gene上的ID一致,不然siRNA序列的具体位置会有差异, BLAST 上的sequence ID为NM_003012.4,sbject为768-778. Gene上的sequence ID为NM_003012.5,sbject为770-790. 当然啦,合适的siRNA序列只是实验成功的一小步,一篇高质量...
Find siRNA sequences - Standard searchInstructions:1. Enter your siRNA name for reference (optional) 2. Paste the sequence of your target gene. (Spaces and numbers will be ignored) 3. Enter the desired motif size (21 nt by default). 4. If you want to increase the siRNA's specificity ...
本例Sequence为: CCGGCGAGATGCTTAAGTGTGACAACTCGAGTTGTCACACTTAAGCATCTCGTTTTTG 所以拟设计的siRNA序列初步确定为黄色高亮部分:CGAGATGCTTAAGTGTGACAA Part 2 巧用NCBI网站验真伪 好了,看到这里是不是觉得设计siRNA序列也就那回事,一条完整的siRNA序列设计已经完成了呢?NO! NO!NO!当siRNA序列初步确定以后,还需要验证...
刚刚记录了siRNA作为dsRNA合成所须的模板:,输入到【Enter accession number(s), gi(s), or FASTA sequence(s)】一栏(图 4.2)。 图4.2 3、选择Database 在Database处选择【Genomic + transcript databases】,因为我们要看设计的siRNA能靶向多少转录本。然后选择对应种属的库【Mouse genomic plus transcript (Mouse...
The invention solves the problem that the prior art has no siRNA sequence which is most appropriate for inhibiting the bak gene expression, the sequence is siRNA1: an antisense strand is 5'-AGUGCACACUUGCUAAAGCtt-3' and a sense strand is 5'-GCUUUAGCAAGUGUGCACUtt-3'; the siRNA double-...
Zhang E, Neugebauer ME, Krasnow NA, Liu DR. Phage-assisted evolution of highly active cytosine base editors with enhanced selectivity and minimal sequence context preference. Nat Commun. 2024 Feb 24;15(1):1697. doi: 1...
Truss, M. et al. HuSiDa–the human siRNA database: an open-access database for published functional siRNA sequences and technical details of efficient transfer into recipient cells. Nucleic Acids Res. 33, D108–D111 (2005). 除此之外,从一些商业来源也可以获得有效的siRNAs(比如Qiagen的HP validat...
Silencer siRNA Libraries are sets of siRNAs targeting groups of genes from functional gene classes to whole genomes, so they are ideal for pathway analysis and target identification and validation. Most feature three individual siRNAs per gene for enhanced data reli...